Site-specific introduction of sialic acid into insulin

Masaaki Sato, Reiko Sadamoto, Kenichi Niikura, Kenji Monde, Hirosato Kondo, Shin Ichiro Nishimura

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

More gain, less pain: A new long-acting insulin (see structure) was created by the enzymatic introduction of sialyllactose into mutant insulins. The glycosylation site was introduced into the insulin by point mutation without loss of biological activity. The experimental results with mice clearly demonstrated that the introduction of a sialic acid residue is crucial in prolonging glucose-lowering activity in the blood.

Original languageEnglish
Pages (from-to)1516-1520
Number of pages5
JournalAngewandte Chemie - International Edition
Volume43
Issue number12
DOIs
Publication statusPublished - Mar 12 2004
Externally publishedYes

Fingerprint

Long-Acting Insulin
Insulins
Glycosylation
Insulin
N-Acetylneuraminic Acid
Bioactivity
Blood
Glucose
Acids
N-acetylneuraminoyllactose

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

Cite this

Sato, M., Sadamoto, R., Niikura, K., Monde, K., Kondo, H., & Nishimura, S. I. (2004). Site-specific introduction of sialic acid into insulin. Angewandte Chemie - International Edition, 43(12), 1516-1520. https://doi.org/10.1002/anie.200353058

Site-specific introduction of sialic acid into insulin. / Sato, Masaaki; Sadamoto, Reiko; Niikura, Kenichi; Monde, Kenji; Kondo, Hirosato; Nishimura, Shin Ichiro.

In: Angewandte Chemie - International Edition, Vol. 43, No. 12, 12.03.2004, p. 1516-1520.

Research output: Contribution to journalArticle

Sato, M, Sadamoto, R, Niikura, K, Monde, K, Kondo, H & Nishimura, SI 2004, 'Site-specific introduction of sialic acid into insulin', Angewandte Chemie - International Edition, vol. 43, no. 12, pp. 1516-1520. https://doi.org/10.1002/anie.200353058
Sato, Masaaki ; Sadamoto, Reiko ; Niikura, Kenichi ; Monde, Kenji ; Kondo, Hirosato ; Nishimura, Shin Ichiro. / Site-specific introduction of sialic acid into insulin. In: Angewandte Chemie - International Edition. 2004 ; Vol. 43, No. 12. pp. 1516-1520.
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