Site-specific introduction of sialic acid into insulin

Masaaki Sato, Reiko Sadamoto, Kenichi Niikura, Kenji Monde, Hirosato Kondo, Shin Ichiro Nishimura

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)


More gain, less pain: A new long-acting insulin (see structure) was created by the enzymatic introduction of sialyllactose into mutant insulins. The glycosylation site was introduced into the insulin by point mutation without loss of biological activity. The experimental results with mice clearly demonstrated that the introduction of a sialic acid residue is crucial in prolonging glucose-lowering activity in the blood.

Original languageEnglish
Pages (from-to)1516-1520
Number of pages5
JournalAngewandte Chemie - International Edition
Issue number12
Publication statusPublished - Mar 12 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)


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