Size exclusion chromatography of standard polystyrenes with a wide range of molecular weight Up to 7.45 × 106 on monolithic silica capillary columns

Koichi Ute, Saori Yoshida, Tatsuki Kitayama, Takeshi Bamba, Kazuo Harada, Eiichiro Fukusaki, Akio Kobayashi, Norio Ishizuka, Hiroyoshi Minakuchi, Kazuki Nakanishi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The separation of polystyrene standards with molecular weight ranging from 2,900 to 7,450,000 by the HPLC using monolithic silica capillaries with a dimension of 200 μm i.d. × 150 cm was investigated. Monolithic silica capillary columns were prepared by hydrolysis and polycondensation of alkoxysilanes in a fused-silica capillary. The polystyrene was dissolved at a concentration of 5g/L in the mobile phase containing a small amount of toluene and for each measurement, 100nL of the solution was injected into each column. The retention time was determined from the peak maximum and converted to the retention volume at a flow rate. Results show that the separation of polymers in the external pore zone of monolithic silica made in capillary (S-B) was more effective than that made as rod (S-A), and as effective as the separation in the internal pore zone.

Original languageEnglish
Pages (from-to)1194-1197
Number of pages4
JournalPolymer Journal
Volume38
Issue number11
DOIs
Publication statusPublished - Dec 1 2006
Externally publishedYes

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Size exclusion chromatography
Polystyrenes
Silicon Dioxide
Molecular weight
Silica
Distillation columns
Toluene
Fused silica
Polycondensation
Hydrolysis
Polymers
Flow rate

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics
  • Materials Chemistry

Cite this

Size exclusion chromatography of standard polystyrenes with a wide range of molecular weight Up to 7.45 × 106 on monolithic silica capillary columns. / Ute, Koichi; Yoshida, Saori; Kitayama, Tatsuki; Bamba, Takeshi; Harada, Kazuo; Fukusaki, Eiichiro; Kobayashi, Akio; Ishizuka, Norio; Minakuchi, Hiroyoshi; Nakanishi, Kazuki.

In: Polymer Journal, Vol. 38, No. 11, 01.12.2006, p. 1194-1197.

Research output: Contribution to journalArticle

Ute, K, Yoshida, S, Kitayama, T, Bamba, T, Harada, K, Fukusaki, E, Kobayashi, A, Ishizuka, N, Minakuchi, H & Nakanishi, K 2006, 'Size exclusion chromatography of standard polystyrenes with a wide range of molecular weight Up to 7.45 × 106 on monolithic silica capillary columns', Polymer Journal, vol. 38, no. 11, pp. 1194-1197. https://doi.org/10.1295/polymj.PJ2006079
Ute, Koichi ; Yoshida, Saori ; Kitayama, Tatsuki ; Bamba, Takeshi ; Harada, Kazuo ; Fukusaki, Eiichiro ; Kobayashi, Akio ; Ishizuka, Norio ; Minakuchi, Hiroyoshi ; Nakanishi, Kazuki. / Size exclusion chromatography of standard polystyrenes with a wide range of molecular weight Up to 7.45 × 106 on monolithic silica capillary columns. In: Polymer Journal. 2006 ; Vol. 38, No. 11. pp. 1194-1197.
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AU - Harada, Kazuo

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