SKP2 inactivation suppresses prostate tumorigenesis by mediating JARID1B ubiquitination

Wenfu Lu, Shenji Liu, Bo Li, Yingqiu Xie, Christine Adhiambo, Qing Yang, Billy R. Ballard, Keiichi I. Nakayama, Robert J. Matusik, Zhenbang Chen

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Aberrant elevation of JARID1B and histone H3 lysine 4 trimethylation (H3K4me3) is frequently observed in many diseases including prostate cancer (PCa), yet the mechanisms on the regulation of JARID1B and H3K4me3 through epigenetic alterations still remain poorly understood. Here we report that Skp2 modulates JARID1B and H3K4me3 levels in vitro in cultured cells and in vivo in mouse models. We demonstrated that Skp2 inactivation decreased H3K4me3 levels, along with a reduction of cell growth, cell migration and malignant transformation of Pten/Trp53 double null MEFs, and further restrained prostate tumorigenesis of Pten/Trp53 mutant mice. Mechanistically, Skp2 decreased the K63-linked ubiquitination of JARID1B by E3 ubiquitin ligase TRAF6, thus decreasing JARID1B demethylase activity and in turn increasing H3K4me3. In agreement, Skp2 deficiency resulted in an increase of JARID1B ubiquitination and in turn a reduction of H3K4me3, and induced senescence through JARID1B accumulation in nucleoli of PCa cells and prostate tumors of mice. Furthermore, we showed that the elevations of Skp2 and H3K4me3 contributed to castration-resistant prostate cancer (CRPC) in mice, and were positively correlated in human PCa specimens. Taken together, our findings reveal a novel network of SKP2- JARID1B, and targeting SKP2 and JARID1B may be a potential strategy for PCa control.

Original languageEnglish
Pages (from-to)771-788
Number of pages18
JournalOncotarget
Volume6
Issue number2
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Ubiquitination
Prostate
Prostatic Neoplasms
Carcinogenesis
TNF Receptor-Associated Factor 6
Ubiquitin-Protein Ligases
Castration
Epigenomics
Histones
Lysine
Cell Movement
Cultured Cells
Growth
Neoplasms

All Science Journal Classification (ASJC) codes

  • Oncology

Cite this

Lu, W., Liu, S., Li, B., Xie, Y., Adhiambo, C., Yang, Q., ... Chen, Z. (2015). SKP2 inactivation suppresses prostate tumorigenesis by mediating JARID1B ubiquitination. Oncotarget, 6(2), 771-788. https://doi.org/10.18632/oncotarget.2718

SKP2 inactivation suppresses prostate tumorigenesis by mediating JARID1B ubiquitination. / Lu, Wenfu; Liu, Shenji; Li, Bo; Xie, Yingqiu; Adhiambo, Christine; Yang, Qing; Ballard, Billy R.; Nakayama, Keiichi I.; Matusik, Robert J.; Chen, Zhenbang.

In: Oncotarget, Vol. 6, No. 2, 01.01.2015, p. 771-788.

Research output: Contribution to journalArticle

Lu, W, Liu, S, Li, B, Xie, Y, Adhiambo, C, Yang, Q, Ballard, BR, Nakayama, KI, Matusik, RJ & Chen, Z 2015, 'SKP2 inactivation suppresses prostate tumorigenesis by mediating JARID1B ubiquitination', Oncotarget, vol. 6, no. 2, pp. 771-788. https://doi.org/10.18632/oncotarget.2718
Lu, Wenfu ; Liu, Shenji ; Li, Bo ; Xie, Yingqiu ; Adhiambo, Christine ; Yang, Qing ; Ballard, Billy R. ; Nakayama, Keiichi I. ; Matusik, Robert J. ; Chen, Zhenbang. / SKP2 inactivation suppresses prostate tumorigenesis by mediating JARID1B ubiquitination. In: Oncotarget. 2015 ; Vol. 6, No. 2. pp. 771-788.
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