SLAM (CD150)-Independent measles virus entry as revealed by recombinant virus expressing green fluorescent protein

Koji Hashimoto, Nobuyuki Ono, Hironobu Tatsuo, Hiroko Minagawa, Makoto Takeda, Kaoru Takeuchi, Yusuke Yanagi

Research output: Contribution to journalArticle

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Abstract

Wild-type measles virus (MV) strains use human signaling lymphocyte activation molecule (SLAM) as a cellular receptor, while vaccine strains such as the Edmonston strain can use both SLAM and CD46 as receptors. Although the expression of SLAM is restricted to cells of the immune system (lymphocytes, dendritic cells, and monocytes), histopathological studies with humans and experimentally infected monkeys have shown that MV also infects SLAM-negative cells, including epithelial, endothelial, and neuronal cells. In an attempt to explain these findings, we produced the enhanced green fluorescent protein (EGFP)-expressing recombinant MV (IC323-EGFP) based on the wild-type IC-B strain. IC323-EGFP showed almost the same growth kinetics as the parental recombinant MV and produced large syncytia exhibiting green autofluorescence in SLAM positive cells. Interestingly, all SLAM-negative cell lines examined also showed green autofluorescence after infection with IC323-EGFP, although the virus hardly spread from the originally infected individual cells and thus did not induce syncytia. When the number of EGFP-expressing cells after infection was taken as an indicator, the infectivities of IC323-EGFP for SLAM-negative cells were 2 to 3 logs lower than those for SLAM-positive cells. Anti-MV hemagglutinin antibody or fusion block peptide, but not anti-CD46 antibody, blocked IC323-EGFP infection of SLAM-negative cells. This infection occurred under conditions in which entry via endocytosis was inhibited. These results indicate that MV can infect a variety of cells, albeit with a low efficiency, by using an as yet unidentified receptor(s) other than SLAM or CD46, in part explaining the observed MV infection of SLAM-negative cells in vivo.

Original languageEnglish
Pages (from-to)6743-6749
Number of pages7
JournalJournal of virology
Volume76
Issue number13
DOIs
Publication statusPublished - Jun 25 2002

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Measles virus
Virus Internalization
lymphocyte proliferation
Lymphocyte Activation
Green Fluorescent Proteins
green fluorescent protein
Viruses
viruses
cells
infection
giant cells
Giant Cells
Infection
receptors
antibodies
Hemagglutinins
endocytosis
Virus Diseases
hemagglutinins
dendritic cells

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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SLAM (CD150)-Independent measles virus entry as revealed by recombinant virus expressing green fluorescent protein. / Hashimoto, Koji; Ono, Nobuyuki; Tatsuo, Hironobu; Minagawa, Hiroko; Takeda, Makoto; Takeuchi, Kaoru; Yanagi, Yusuke.

In: Journal of virology, Vol. 76, No. 13, 25.06.2002, p. 6743-6749.

Research output: Contribution to journalArticle

Hashimoto, Koji ; Ono, Nobuyuki ; Tatsuo, Hironobu ; Minagawa, Hiroko ; Takeda, Makoto ; Takeuchi, Kaoru ; Yanagi, Yusuke. / SLAM (CD150)-Independent measles virus entry as revealed by recombinant virus expressing green fluorescent protein. In: Journal of virology. 2002 ; Vol. 76, No. 13. pp. 6743-6749.
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