Slow-Myofiber Commitment by Semaphorin 3A Secreted from Myogenic Stem Cells

Ryuichi Tatsumi, Takahiro Suzuki, Mai Khoi Q. Do, Yuki Ohya, Judy E. Anderson, Ayumi Shibata, Mai Kawaguchi, Shunpei Ohya, Hideaki Ohtsubo, Wataru Mizunoya, Shoko Sawano, Yusuke Komiya, Riho Ichitsubo, Koichi Ojima, Shin Ichiro Nishimatsu, Tsutomu Nohno, Yutaka Ohsawa, Yoshihide Sunada, Mako Nakamura, Mitsuhiro Furuse & 4 others Yoshihide Ikeuchi, Takanori Nishimura, Takeshi Yagi, Ronald E. Allen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Recently, we found that resident myogenic stem satellite cells upregulate a multi-functional secreted protein, semaphorin 3A (Sema3A), exclusively at the early-differentiation phase in response to muscle injury; however, its physiological significance is still unknown. Here we show that Sema3A impacts slow-twitch fiber generation through a signaling pathway, cell-membrane receptor (neuropilin2-plexinA3) → myogenin-myocyte enhancer factor 2D → slow myosin heavy chain. This novel axis was found by small interfering RNA-transfection experiments in myoblast cultures, which also revealed an additional element that Sema3A-neuropilin1/plexinA1, A2 may enhance slow-fiber formation by activating signals that inhibit fast-myosin expression. Importantly, satellite cell-specific Sema3A conditional-knockout adult mice (Pax7CreERT2-Sema3Afl°x activated by tamoxifen-i.p. injection) provided direct in vivo evidence for the Sema3A-driven program, by showing that slow-fiber generation and muscle endurance were diminished after repair from cardiotoxin-injury of gastrocnemius muscle. Overall, the findings highlight an active role for satellite cell-secreted Sema3A ligand as a key “commitment factor” for the slow-fiber population during muscle regeneration. Results extend our understanding of the myogenic stem-cell strategy that regulates fiber-type differentiation and is responsible for skeletal muscle contractility, energy metabolism, fatigue resistance, and its susceptibility to aging and disease. Stem Cells 2017;35:1815–1834.

Original languageEnglish
Pages (from-to)1815-1834
Number of pages20
JournalSTEM CELLS
Volume35
Issue number7
DOIs
Publication statusPublished - Jul 1 2017

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Semaphorin-3A
Stem Cells
Muscles
Skeletal Muscle
Skeletal Muscle Satellite Cells
Cardiotoxins
MEF2 Transcription Factors
Myogenin
Myosin Heavy Chains
Myoblasts
Wounds and Injuries
Tamoxifen
Myosins
varespladib methyl
Knockout Mice
Energy Metabolism
Small Interfering RNA
Fatigue
Transfection
Regeneration

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Tatsumi, R., Suzuki, T., Do, M. K. Q., Ohya, Y., Anderson, J. E., Shibata, A., ... Allen, R. E. (2017). Slow-Myofiber Commitment by Semaphorin 3A Secreted from Myogenic Stem Cells. STEM CELLS, 35(7), 1815-1834. https://doi.org/10.1002/stem.2639

Slow-Myofiber Commitment by Semaphorin 3A Secreted from Myogenic Stem Cells. / Tatsumi, Ryuichi; Suzuki, Takahiro; Do, Mai Khoi Q.; Ohya, Yuki; Anderson, Judy E.; Shibata, Ayumi; Kawaguchi, Mai; Ohya, Shunpei; Ohtsubo, Hideaki; Mizunoya, Wataru; Sawano, Shoko; Komiya, Yusuke; Ichitsubo, Riho; Ojima, Koichi; Nishimatsu, Shin Ichiro; Nohno, Tsutomu; Ohsawa, Yutaka; Sunada, Yoshihide; Nakamura, Mako; Furuse, Mitsuhiro; Ikeuchi, Yoshihide; Nishimura, Takanori; Yagi, Takeshi; Allen, Ronald E.

In: STEM CELLS, Vol. 35, No. 7, 01.07.2017, p. 1815-1834.

Research output: Contribution to journalArticle

Tatsumi, R, Suzuki, T, Do, MKQ, Ohya, Y, Anderson, JE, Shibata, A, Kawaguchi, M, Ohya, S, Ohtsubo, H, Mizunoya, W, Sawano, S, Komiya, Y, Ichitsubo, R, Ojima, K, Nishimatsu, SI, Nohno, T, Ohsawa, Y, Sunada, Y, Nakamura, M, Furuse, M, Ikeuchi, Y, Nishimura, T, Yagi, T & Allen, RE 2017, 'Slow-Myofiber Commitment by Semaphorin 3A Secreted from Myogenic Stem Cells', STEM CELLS, vol. 35, no. 7, pp. 1815-1834. https://doi.org/10.1002/stem.2639
Tatsumi R, Suzuki T, Do MKQ, Ohya Y, Anderson JE, Shibata A et al. Slow-Myofiber Commitment by Semaphorin 3A Secreted from Myogenic Stem Cells. STEM CELLS. 2017 Jul 1;35(7):1815-1834. https://doi.org/10.1002/stem.2639
Tatsumi, Ryuichi ; Suzuki, Takahiro ; Do, Mai Khoi Q. ; Ohya, Yuki ; Anderson, Judy E. ; Shibata, Ayumi ; Kawaguchi, Mai ; Ohya, Shunpei ; Ohtsubo, Hideaki ; Mizunoya, Wataru ; Sawano, Shoko ; Komiya, Yusuke ; Ichitsubo, Riho ; Ojima, Koichi ; Nishimatsu, Shin Ichiro ; Nohno, Tsutomu ; Ohsawa, Yutaka ; Sunada, Yoshihide ; Nakamura, Mako ; Furuse, Mitsuhiro ; Ikeuchi, Yoshihide ; Nishimura, Takanori ; Yagi, Takeshi ; Allen, Ronald E. / Slow-Myofiber Commitment by Semaphorin 3A Secreted from Myogenic Stem Cells. In: STEM CELLS. 2017 ; Vol. 35, No. 7. pp. 1815-1834.
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