Slowly dividing neural progenitors are an embryonic origin of adult neural stem cells

Shohei Furutachi, Hiroaki Miya, Tomoyuki Watanabe, Hiroki Kawai, Norihiko Yamasaki, Yujin Harada, Itaru Imayoshi, Mark Nelson, Keiichi Nakayama, Yusuke Hirabayashi, Yukiko Gotoh

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

The mechanism by which adult neural stem cells (NSCs) are established during development is unclear. In this study, analysis of cell cycle progression by examining retention of a histone 2B (H2B)-GFP fusion protein revealed that, in a subset of mouse embryonic neural progenitor cells (NPCs), the cell cycle slows between embryonic day (E) 13.5 and E15.5 while other embryonic NPCs continue to divide rapidly. By allowing H2B-GFP expressed at E9.5 to become diluted in dividing cells until the young adult stage, we determined that a majority of NSCs in the young adult subependymal zone (SEZ) originated from these slowly dividing embryonic NPCs. The cyclin-dependent kinase inhibitor p57 is highly expressed in this embryonic subpopulation, and the deletion of p57 impairs the emergence of adult NSCs. Our results suggest that a substantial fraction of adult SEZ NSCs is derived from a slowly dividing subpopulation of embryonic NPCs and identify p57 as a key factor in generating this embryonic origin of adult SEZ NSCs.

Original languageEnglish
Pages (from-to)657-665
Number of pages9
JournalNature Neuroscience
Volume18
Issue number5
DOIs
Publication statusPublished - Apr 28 2015

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Adult Stem Cells
Neural Stem Cells
Stem Cells
Histones
Cyclin-Dependent Kinase Inhibitor p57
Young Adult
Cell Cycle
Proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Furutachi, S., Miya, H., Watanabe, T., Kawai, H., Yamasaki, N., Harada, Y., ... Gotoh, Y. (2015). Slowly dividing neural progenitors are an embryonic origin of adult neural stem cells. Nature Neuroscience, 18(5), 657-665. https://doi.org/10.1038/nn.3989

Slowly dividing neural progenitors are an embryonic origin of adult neural stem cells. / Furutachi, Shohei; Miya, Hiroaki; Watanabe, Tomoyuki; Kawai, Hiroki; Yamasaki, Norihiko; Harada, Yujin; Imayoshi, Itaru; Nelson, Mark; Nakayama, Keiichi; Hirabayashi, Yusuke; Gotoh, Yukiko.

In: Nature Neuroscience, Vol. 18, No. 5, 28.04.2015, p. 657-665.

Research output: Contribution to journalArticle

Furutachi, S, Miya, H, Watanabe, T, Kawai, H, Yamasaki, N, Harada, Y, Imayoshi, I, Nelson, M, Nakayama, K, Hirabayashi, Y & Gotoh, Y 2015, 'Slowly dividing neural progenitors are an embryonic origin of adult neural stem cells', Nature Neuroscience, vol. 18, no. 5, pp. 657-665. https://doi.org/10.1038/nn.3989
Furutachi S, Miya H, Watanabe T, Kawai H, Yamasaki N, Harada Y et al. Slowly dividing neural progenitors are an embryonic origin of adult neural stem cells. Nature Neuroscience. 2015 Apr 28;18(5):657-665. https://doi.org/10.1038/nn.3989
Furutachi, Shohei ; Miya, Hiroaki ; Watanabe, Tomoyuki ; Kawai, Hiroki ; Yamasaki, Norihiko ; Harada, Yujin ; Imayoshi, Itaru ; Nelson, Mark ; Nakayama, Keiichi ; Hirabayashi, Yusuke ; Gotoh, Yukiko. / Slowly dividing neural progenitors are an embryonic origin of adult neural stem cells. In: Nature Neuroscience. 2015 ; Vol. 18, No. 5. pp. 657-665.
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