Small circular single stranded DNA viral genomes in unexplained cases of human encephalitis, diarrhea, and in untreated sewage

Tung Gia Phan; Daisuke Mori; Xutao Deng; Shaman Rajidrajith; Udaya Ranawaka; Terry Fei Fan Ng; Filemon Bucardo-Rivera; Patricia Orlandi; Kamruddin Ahmed; Eric Delwart

doi:10.1016/j.virol.2015.03.011

Small circular single stranded DNA viral genomes in unexplained cases of human encephalitis, diarrhea, and in untreated sewage

Tung Gia Phan, Daisuke Mori, Xutao Deng, Shaman Rajidrajith, Udaya Ranawaka, Terry Fei Fan Ng, Filemon Bucardo-Rivera, Patricia Orlandi, Kamruddin Ahmed, Eric Delwart

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

Viruses with small circular ssDNA genomes encoding a replication initiator protein can infect a wide range of eukaryotic organisms ranging from mammals to fungi. The genomes of two such viruses, a cyclovirus (CyCV-SL) and gemycircularvirus (GemyCV-SL) were detected by deep sequencing of the cerebrospinal fluids of Sri Lankan patients with unexplained encephalitis. One and three out of 201 CSF samples (1.5%) from unexplained encephalitis patients tested by PCR were CyCV-SL and GemyCV-SL DNA positive respectively. Nucleotide similarity searches of pre-existing metagenomics datasets revealed closely related genomes in feces from unexplained cases of diarrhea from Nicaragua and Brazil and in untreated sewage from Nepal. Whether the tropism of the cyclovirus and gemycircularvirus reported here include humans or other cellular sources in or on the human body remains to be determined.

Original language	English
Pages (from-to)	98-104
Number of pages	7
Journal	Virology
Volume	482
DOIs	https://doi.org/10.1016/j.virol.2015.03.011
Publication status	Published - Aug 1 2015
Externally published	Yes

All Science Journal Classification (ASJC) codes

Virology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virol.2015.03.011

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title = "Small circular single stranded DNA viral genomes in unexplained cases of human encephalitis, diarrhea, and in untreated sewage",

abstract = "Viruses with small circular ssDNA genomes encoding a replication initiator protein can infect a wide range of eukaryotic organisms ranging from mammals to fungi. The genomes of two such viruses, a cyclovirus (CyCV-SL) and gemycircularvirus (GemyCV-SL) were detected by deep sequencing of the cerebrospinal fluids of Sri Lankan patients with unexplained encephalitis. One and three out of 201 CSF samples (1.5%) from unexplained encephalitis patients tested by PCR were CyCV-SL and GemyCV-SL DNA positive respectively. Nucleotide similarity searches of pre-existing metagenomics datasets revealed closely related genomes in feces from unexplained cases of diarrhea from Nicaragua and Brazil and in untreated sewage from Nepal. Whether the tropism of the cyclovirus and gemycircularvirus reported here include humans or other cellular sources in or on the human body remains to be determined.",

author = "Phan, {Tung Gia} and Daisuke Mori and Xutao Deng and Shaman Rajidrajith and Udaya Ranawaka and {Fan Ng}, {Terry Fei} and Filemon Bucardo-Rivera and Patricia Orlandi and Kamruddin Ahmed and Eric Delwart",

note = "Funding Information: This work was supported by NHLBI Grant R01 HL105770 and support from the Blood Systems Research Institute to E. D. and a Research Fund at the Discretion of the President, Oita University (Grant no. 610000-N5021 ) to K.A. Publisher Copyright: {\textcopyright} 2015 Elsevier Inc.",

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doi = "10.1016/j.virol.2015.03.011",

language = "English",

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AU - Phan, Tung Gia

AU - Mori, Daisuke

AU - Deng, Xutao

AU - Rajidrajith, Shaman

AU - Ranawaka, Udaya

AU - Fan Ng, Terry Fei

AU - Bucardo-Rivera, Filemon

AU - Orlandi, Patricia

AU - Ahmed, Kamruddin

AU - Delwart, Eric

N1 - Funding Information: This work was supported by NHLBI Grant R01 HL105770 and support from the Blood Systems Research Institute to E. D. and a Research Fund at the Discretion of the President, Oita University (Grant no. 610000-N5021 ) to K.A. Publisher Copyright: © 2015 Elsevier Inc.

PY - 2015/8/1

Y1 - 2015/8/1

N2 - Viruses with small circular ssDNA genomes encoding a replication initiator protein can infect a wide range of eukaryotic organisms ranging from mammals to fungi. The genomes of two such viruses, a cyclovirus (CyCV-SL) and gemycircularvirus (GemyCV-SL) were detected by deep sequencing of the cerebrospinal fluids of Sri Lankan patients with unexplained encephalitis. One and three out of 201 CSF samples (1.5%) from unexplained encephalitis patients tested by PCR were CyCV-SL and GemyCV-SL DNA positive respectively. Nucleotide similarity searches of pre-existing metagenomics datasets revealed closely related genomes in feces from unexplained cases of diarrhea from Nicaragua and Brazil and in untreated sewage from Nepal. Whether the tropism of the cyclovirus and gemycircularvirus reported here include humans or other cellular sources in or on the human body remains to be determined.

AB - Viruses with small circular ssDNA genomes encoding a replication initiator protein can infect a wide range of eukaryotic organisms ranging from mammals to fungi. The genomes of two such viruses, a cyclovirus (CyCV-SL) and gemycircularvirus (GemyCV-SL) were detected by deep sequencing of the cerebrospinal fluids of Sri Lankan patients with unexplained encephalitis. One and three out of 201 CSF samples (1.5%) from unexplained encephalitis patients tested by PCR were CyCV-SL and GemyCV-SL DNA positive respectively. Nucleotide similarity searches of pre-existing metagenomics datasets revealed closely related genomes in feces from unexplained cases of diarrhea from Nicaragua and Brazil and in untreated sewage from Nepal. Whether the tropism of the cyclovirus and gemycircularvirus reported here include humans or other cellular sources in or on the human body remains to be determined.

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JO - Virology

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Small circular single stranded DNA viral genomes in unexplained cases of human encephalitis, diarrhea, and in untreated sewage

Abstract

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