Small GTP-binding protein, Rab6, is associated with secretory granules in atrial myocytes

Hiroshi Iida, Shigeyasu Tanaka, Yosaburo Shibata

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Rab proteins, a subfamily of small GTP-binding proteins, have been shown to play key roles in regulation of vesicular traffic in eukaryotic cells. In this study, we have intended to identify the atrial granule-associated Rab proteins that seem to be required for formation or intracellular transport of the granules. Atrial granules contained at least four small GTP-binding proteins, and we have demonstrated by biochemical analysis that one of the small GTP-binding proteins associated with the atrial granules is a Rab6 protein (Rab6p). Rab6p was also detected in highly purified zymogen granules of pancreatic exocrine gland. Immunogold electron microscopy performed on ultrathin cryosections of rat auricle revealed that Rab6p was associated with the atrial granule membranes. Association of Rab6p with the atrial granule membranes was also confirmed by immunodiffusion electron microscopy in agarose-embedded atrial granules. These data indicate that Rab6p is associated with the atrial granules and that it might function in the intracellular traffic of the secretory granules in the atrial myocytes.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume272
Issue number5 41-5
Publication statusPublished - Jun 26 1997

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Secretory Vesicles
GTP-Binding Proteins
Muscle Cells
Electron Microscopy
Exocrine Glands
Membranes
Immunodiffusion
Eukaryotic Cells
Sepharose
Proteins
Rab6 protein

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cell Biology

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Small GTP-binding protein, Rab6, is associated with secretory granules in atrial myocytes. / Iida, Hiroshi; Tanaka, Shigeyasu; Shibata, Yosaburo.

In: American Journal of Physiology - Cell Physiology, Vol. 272, No. 5 41-5, 26.06.1997.

Research output: Contribution to journalArticle

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