TY - CHAP
T1 - Small-molecule screening to increase agrobacterium-mediated transformation efficiency in legumes
AU - Kimura, Mitsuhiro
AU - Isobe, Sachiko
N1 - Publisher Copyright:
© 2018, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2018
Y1 - 2018
N2 - Agrobacterium-mediated transformation is a powerful strategy for plant genetic engineering. However, the transformation efficiency of some grain legume crops is generally low. In this chapter, we describe a chemical screening procedure for improving the efficiency of Agrobacterium-mediated transformation of a model legume, Lotus japonicus. Moreover, we explain the transformation protocol using chloroxynil, a phenolic compound that improves the efficiency.
AB - Agrobacterium-mediated transformation is a powerful strategy for plant genetic engineering. However, the transformation efficiency of some grain legume crops is generally low. In this chapter, we describe a chemical screening procedure for improving the efficiency of Agrobacterium-mediated transformation of a model legume, Lotus japonicus. Moreover, we explain the transformation protocol using chloroxynil, a phenolic compound that improves the efficiency.
UR - http://www.scopus.com/inward/record.url?scp=85048027272&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85048027272&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7874-8_8
DO - 10.1007/978-1-4939-7874-8_8
M3 - Chapter
C2 - 29846921
AN - SCOPUS:85048027272
T3 - Methods in Molecular Biology
SP - 93
EP - 99
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -