Small RNA class transition from siRNA/piRNA to miRNA during pre-implantation mouse development

Yusuke Ohnishi, Yasushi Totoki, Atsushi Toyoda, Toshiaki Watanabe, Yasuhiro Yamamoto, Katsushi Tokunaga, Yoshiyuki Sakaki, Hiroyuki Sasaki, Hirohiko Hohjoh

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Recent studies showed that small interfering RNAs (siRNAs) and Piwi-interacting RNA (piRNA) in mammalian germ cells play important roles in retrotransposon silencing and gametogenesis. However, subsequent contribution of those small RNAs to early mammalian development remains poorly understood. We investigated the expression profiles of small RNAs in mouse metaphase II oocytes, 8-16-cell stage embryos, blastocysts and the pluripotent inner cell mass (ICM) using high-throughput pyrosequencing. Here, we show that during pre-implantation development a major small RNA class changes from retrotransposon-derived small RNAs containing siRNAs and piRNAs to zygotically synthesized microRNAs (miRNAs). Some siRNAs and piRNAs are transiently upregulated and directed against specific retrotransposon classes. We also identified miRNAs expression profiles characteristic of the ICM and trophectoderm (TE) cells. Taken together, our current study reveals a major reprogramming of functional small RNAs during early mouse development from oocyte to blastocyst.

Original languageEnglish
Article numbergkq229
Pages (from-to)5141-5151
Number of pages11
JournalNucleic acids research
Volume38
Issue number15
DOIs
Publication statusPublished - Apr 12 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

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    Ohnishi, Y., Totoki, Y., Toyoda, A., Watanabe, T., Yamamoto, Y., Tokunaga, K., Sakaki, Y., Sasaki, H., & Hohjoh, H. (2010). Small RNA class transition from siRNA/piRNA to miRNA during pre-implantation mouse development. Nucleic acids research, 38(15), 5141-5151. [gkq229]. https://doi.org/10.1093/nar/gkq229