Small RNA class transition from siRNA/piRNA to miRNA during pre-implantation mouse development

Yusuke Ohnishi; Yasushi Totoki; Atsushi Toyoda; Toshiaki Watanabe; Yasuhiro Yamamoto; Katsushi Tokunaga; Yoshiyuki Sakaki; Hiroyuki Sasaki; Hirohiko Hohjoh

doi:10.1093/nar/gkq229

Small RNA class transition from siRNA/piRNA to miRNA during pre-implantation mouse development

Yusuke Ohnishi, Yasushi Totoki, Atsushi Toyoda, Toshiaki Watanabe, Yasuhiro Yamamoto, Katsushi Tokunaga, Yoshiyuki Sakaki, Hiroyuki Sasaki, Hirohiko Hohjoh

Research output: Contribution to journal › Article › peer-review

109 Citations (Scopus)

Abstract

Recent studies showed that small interfering RNAs (siRNAs) and Piwi-interacting RNA (piRNA) in mammalian germ cells play important roles in retrotransposon silencing and gametogenesis. However, subsequent contribution of those small RNAs to early mammalian development remains poorly understood. We investigated the expression profiles of small RNAs in mouse metaphase II oocytes, 8-16-cell stage embryos, blastocysts and the pluripotent inner cell mass (ICM) using high-throughput pyrosequencing. Here, we show that during pre-implantation development a major small RNA class changes from retrotransposon-derived small RNAs containing siRNAs and piRNAs to zygotically synthesized microRNAs (miRNAs). Some siRNAs and piRNAs are transiently upregulated and directed against specific retrotransposon classes. We also identified miRNAs expression profiles characteristic of the ICM and trophectoderm (TE) cells. Taken together, our current study reveals a major reprogramming of functional small RNAs during early mouse development from oocyte to blastocyst.

Original language	English
Article number	gkq229
Pages (from-to)	5141-5151
Number of pages	11
Journal	Nucleic acids research
Volume	38
Issue number	15
DOIs	https://doi.org/10.1093/nar/gkq229
Publication status	Published - Apr 12 2010

All Science Journal Classification (ASJC) codes

Genetics

Access to Document

10.1093/nar/gkq229

Cite this

@article{671b53241f884a0a88be2a2118cc5667,

title = "Small RNA class transition from siRNA/piRNA to miRNA during pre-implantation mouse development",

abstract = "Recent studies showed that small interfering RNAs (siRNAs) and Piwi-interacting RNA (piRNA) in mammalian germ cells play important roles in retrotransposon silencing and gametogenesis. However, subsequent contribution of those small RNAs to early mammalian development remains poorly understood. We investigated the expression profiles of small RNAs in mouse metaphase II oocytes, 8-16-cell stage embryos, blastocysts and the pluripotent inner cell mass (ICM) using high-throughput pyrosequencing. Here, we show that during pre-implantation development a major small RNA class changes from retrotransposon-derived small RNAs containing siRNAs and piRNAs to zygotically synthesized microRNAs (miRNAs). Some siRNAs and piRNAs are transiently upregulated and directed against specific retrotransposon classes. We also identified miRNAs expression profiles characteristic of the ICM and trophectoderm (TE) cells. Taken together, our current study reveals a major reprogramming of functional small RNAs during early mouse development from oocyte to blastocyst.",

author = "Yusuke Ohnishi and Yasushi Totoki and Atsushi Toyoda and Toshiaki Watanabe and Yasuhiro Yamamoto and Katsushi Tokunaga and Yoshiyuki Sakaki and Hiroyuki Sasaki and Hirohiko Hohjoh",

note = "Funding Information: Ministry of Health, Labour and Welfare of Japan, Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science and National Institute of Genetics (NIG) Cooperative Research Program (2008-A60). Funding for open access charge: Ministry of Health, Labour and Welfare of Japan.",

year = "2010",

month = apr,

day = "12",

doi = "10.1093/nar/gkq229",

language = "English",

volume = "38",

pages = "5141--5151",

journal = "Nucleic Acids Research",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "15",

}

TY - JOUR

T1 - Small RNA class transition from siRNA/piRNA to miRNA during pre-implantation mouse development

AU - Ohnishi, Yusuke

AU - Totoki, Yasushi

AU - Toyoda, Atsushi

AU - Watanabe, Toshiaki

AU - Yamamoto, Yasuhiro

AU - Tokunaga, Katsushi

AU - Sakaki, Yoshiyuki

AU - Sasaki, Hiroyuki

AU - Hohjoh, Hirohiko

N1 - Funding Information: Ministry of Health, Labour and Welfare of Japan, Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science and National Institute of Genetics (NIG) Cooperative Research Program (2008-A60). Funding for open access charge: Ministry of Health, Labour and Welfare of Japan.

PY - 2010/4/12

Y1 - 2010/4/12

N2 - Recent studies showed that small interfering RNAs (siRNAs) and Piwi-interacting RNA (piRNA) in mammalian germ cells play important roles in retrotransposon silencing and gametogenesis. However, subsequent contribution of those small RNAs to early mammalian development remains poorly understood. We investigated the expression profiles of small RNAs in mouse metaphase II oocytes, 8-16-cell stage embryos, blastocysts and the pluripotent inner cell mass (ICM) using high-throughput pyrosequencing. Here, we show that during pre-implantation development a major small RNA class changes from retrotransposon-derived small RNAs containing siRNAs and piRNAs to zygotically synthesized microRNAs (miRNAs). Some siRNAs and piRNAs are transiently upregulated and directed against specific retrotransposon classes. We also identified miRNAs expression profiles characteristic of the ICM and trophectoderm (TE) cells. Taken together, our current study reveals a major reprogramming of functional small RNAs during early mouse development from oocyte to blastocyst.

AB - Recent studies showed that small interfering RNAs (siRNAs) and Piwi-interacting RNA (piRNA) in mammalian germ cells play important roles in retrotransposon silencing and gametogenesis. However, subsequent contribution of those small RNAs to early mammalian development remains poorly understood. We investigated the expression profiles of small RNAs in mouse metaphase II oocytes, 8-16-cell stage embryos, blastocysts and the pluripotent inner cell mass (ICM) using high-throughput pyrosequencing. Here, we show that during pre-implantation development a major small RNA class changes from retrotransposon-derived small RNAs containing siRNAs and piRNAs to zygotically synthesized microRNAs (miRNAs). Some siRNAs and piRNAs are transiently upregulated and directed against specific retrotransposon classes. We also identified miRNAs expression profiles characteristic of the ICM and trophectoderm (TE) cells. Taken together, our current study reveals a major reprogramming of functional small RNAs during early mouse development from oocyte to blastocyst.

UR - http://www.scopus.com/inward/record.url?scp=77956117446&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956117446&partnerID=8YFLogxK

U2 - 10.1093/nar/gkq229

DO - 10.1093/nar/gkq229

M3 - Article

C2 - 20385573

AN - SCOPUS:77956117446

SN - 0305-1048

VL - 38

SP - 5141

EP - 5151

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 15

M1 - gkq229

ER -

Small RNA class transition from siRNA/piRNA to miRNA during pre-implantation mouse development

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this