@article{ebc39bebda204705889f8662f1efc4ff,
title = "Small synthetic molecule-stabilized RNA pseudoknot as an activator for -1 ribosomal frameshifting",
abstract = "Programmed -1 ribosomal frameshifting (−1PRF) is a recoding mechanism to make alternative proteins from a single mRNA transcript. −1PRF is stimulated by cis-acting signals in mRNA, a seven-nucleotide slippery sequence and a downstream secondary structure element, which is often a pseudoknot. In this study we engineered the frameshifting pseudoknot from the mouse mammary tumor virus to respond to a rationally designed small molecule naphthyridine carbamate tetramer (NCTn). We demonstrate that NCTn can stabilize the pseudoknot structure in mRNA and activate -1PRF both in vitro and in human cells. The results illustrate how NCTn-inducible -1PRF may serve as an important component of the synthetic biology toolbox for the precise control of gene expression using small synthetic molecules.",
author = "Saki Matsumoto and Neva Caliskan and Rodnina, {Marina V.} and Asako Murata and Kazuhiko Nakatani",
note = "Funding Information: JSPS KAKENHI Grant-in-Aid for Specially Promoted Research [26000007 to K.N.]; JSPS KAKENHI Grant-in-Aid for Scientific Research (C) [15K01820 to A.M.]; JSPS KAKENHI Grant-in-Aid for JSPS Fellows [265460 to S.M.]; Research Program of {\textquoteleft}Dynamic Alliance for Open Innovation Bridging Human, Environment and Materials{\textquoteright} in {\textquoteleft}Network Joint Research Center for Materials and Devices{\textquoteright} (in part); Max Planck Society and the Deutsche Forschungsgemeinschaft [SFB 860 to M.V.R]. Funding for open access charge: JSPS KAKENHI Grant-in-Aid for Specially Promoted Research [26000007 to K.N.]; JSPS KAKENHI Grant-in-Aid for Scientific Research (C) [15K01820 to A.M.]. Conflict of interest statement. None declared. Publisher Copyright: {\textcopyright} The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.",
year = "2018",
month = sep,
day = "19",
doi = "10.1093/nar/gky689",
language = "English",
volume = "46",
pages = "8079--8089",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "16",
}