TY - JOUR
T1 - smyd1 and smyd2 are expressed in muscle tissue in Xenopus laevis
AU - Kawamura, Shinobu
AU - Yoshigai, Emi
AU - Kuhara, Satoru
AU - Tashiro, Kosuke
N1 - Funding Information:
Acknowledgements We thank Dr. William Watt for critical reading of manuscripts and Dr. Kaori Kubota for technical advices. This research was supported by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan.
PY - 2008/6
Y1 - 2008/6
N2 - Epigenetic modifications of histone play important roles for regulation of cell activity, such as cell division, cell death, and cell differentiation. A SET domain consisting of about 130 amino acids has lysine methyltransferase activity in the presence of the cosubstrate S-adenosyl-methionine. More than 60 SET domain-containing proteins have been predicted in various organisms. One of them, the SMYD family genes which contain a SET domain and a zinc-finger MYND domain are reported to regulate cell cycle and muscle formation. Here we examined the expression and function of smyd1 and 2 in Xenopus. smyd1 and 2 were expressed in various muscle tissues. While smyd1 expression was observed mainly in cardiac muscle and skeletal muscle, smyd2 expression was done abundantly in skeletal muscle and face region. Moreover, by loss-of-function experiments using antisense morpholino oligonucleotides, it was suggested that smyd1 and 2 related to muscle cells differentiation.
AB - Epigenetic modifications of histone play important roles for regulation of cell activity, such as cell division, cell death, and cell differentiation. A SET domain consisting of about 130 amino acids has lysine methyltransferase activity in the presence of the cosubstrate S-adenosyl-methionine. More than 60 SET domain-containing proteins have been predicted in various organisms. One of them, the SMYD family genes which contain a SET domain and a zinc-finger MYND domain are reported to regulate cell cycle and muscle formation. Here we examined the expression and function of smyd1 and 2 in Xenopus. smyd1 and 2 were expressed in various muscle tissues. While smyd1 expression was observed mainly in cardiac muscle and skeletal muscle, smyd2 expression was done abundantly in skeletal muscle and face region. Moreover, by loss-of-function experiments using antisense morpholino oligonucleotides, it was suggested that smyd1 and 2 related to muscle cells differentiation.
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U2 - 10.1007/s10616-008-9128-1
DO - 10.1007/s10616-008-9128-1
M3 - Article
C2 - 19003161
AN - SCOPUS:50249122610
SN - 0920-9069
VL - 57
SP - 161
EP - 168
JO - Cytotechnology
JF - Cytotechnology
IS - 2
ER -