Snf1-like protein kinase ssp2 regulates glucose derepression in schizosaccharomyces pombe

Tomohiko Matsuzawa, Yasuko Fujita, Hideki Tohda, Kaoru Takegawa

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The function of two fission yeast genes, SPCC74.03c/ssp2 + and SPAC23H4.02/ppk9 +, encoding an Snf1-like protein kinase were investigated. Deletion of ssp2 + caused a partial defect in glucose derepression of inv1 +,fbp1 +, and gld1 + and in assimilation of sucrose and glycerol, while a mutation in ppk9 + had no apparent effect. Scr1, a transcription factor involved in glucose repression, localized to the nucleus under glucose-rich conditions and to the cytoplasm during glucose starvation in wild-type cells. In contrast, in the ssp2A mutant, Scr1 localized to the nucleus in cells grown in glucose-rich medium as well as in glucose-starved cells. Immunoblot analysis showed that Ssp2 is required for the phosphorylation of Scr1 upon glucose deprivation. Mutation of five putative Ssp2 recognition sites in Scr1 prevented glucose derepression of invertase in glucose-starved cells. These results indicate that Ssp2 regulates phosphorylation and subcellular localization of Scr1 in response to glucose.

Original languageEnglish
Pages (from-to)159-167
Number of pages9
JournalEukaryotic Cell
Volume11
Issue number2
DOIs
Publication statusPublished - Feb 1 2012

Fingerprint

Schizosaccharomyces
Protein Kinases
Glucose
Phosphorylation
beta-Fructofuranosidase
Mutation
Starvation
Cell Nucleus
Glycerol
Sucrose
Cytoplasm
Transcription Factors

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Snf1-like protein kinase ssp2 regulates glucose derepression in schizosaccharomyces pombe. / Matsuzawa, Tomohiko; Fujita, Yasuko; Tohda, Hideki; Takegawa, Kaoru.

In: Eukaryotic Cell, Vol. 11, No. 2, 01.02.2012, p. 159-167.

Research output: Contribution to journalArticle

Matsuzawa, Tomohiko ; Fujita, Yasuko ; Tohda, Hideki ; Takegawa, Kaoru. / Snf1-like protein kinase ssp2 regulates glucose derepression in schizosaccharomyces pombe. In: Eukaryotic Cell. 2012 ; Vol. 11, No. 2. pp. 159-167.
@article{6303670fe56b4517b2f82e9b26fc3c14,
title = "Snf1-like protein kinase ssp2 regulates glucose derepression in schizosaccharomyces pombe",
abstract = "The function of two fission yeast genes, SPCC74.03c/ssp2 + and SPAC23H4.02/ppk9 +, encoding an Snf1-like protein kinase were investigated. Deletion of ssp2 + caused a partial defect in glucose derepression of inv1 +,fbp1 +, and gld1 + and in assimilation of sucrose and glycerol, while a mutation in ppk9 + had no apparent effect. Scr1, a transcription factor involved in glucose repression, localized to the nucleus under glucose-rich conditions and to the cytoplasm during glucose starvation in wild-type cells. In contrast, in the ssp2A mutant, Scr1 localized to the nucleus in cells grown in glucose-rich medium as well as in glucose-starved cells. Immunoblot analysis showed that Ssp2 is required for the phosphorylation of Scr1 upon glucose deprivation. Mutation of five putative Ssp2 recognition sites in Scr1 prevented glucose derepression of invertase in glucose-starved cells. These results indicate that Ssp2 regulates phosphorylation and subcellular localization of Scr1 in response to glucose.",
author = "Tomohiko Matsuzawa and Yasuko Fujita and Hideki Tohda and Kaoru Takegawa",
year = "2012",
month = "2",
day = "1",
doi = "10.1128/EC.05268-11",
language = "English",
volume = "11",
pages = "159--167",
journal = "Eukaryotic Cell",
issn = "1535-9778",
publisher = "American Society for Microbiology",
number = "2",

}

TY - JOUR

T1 - Snf1-like protein kinase ssp2 regulates glucose derepression in schizosaccharomyces pombe

AU - Matsuzawa, Tomohiko

AU - Fujita, Yasuko

AU - Tohda, Hideki

AU - Takegawa, Kaoru

PY - 2012/2/1

Y1 - 2012/2/1

N2 - The function of two fission yeast genes, SPCC74.03c/ssp2 + and SPAC23H4.02/ppk9 +, encoding an Snf1-like protein kinase were investigated. Deletion of ssp2 + caused a partial defect in glucose derepression of inv1 +,fbp1 +, and gld1 + and in assimilation of sucrose and glycerol, while a mutation in ppk9 + had no apparent effect. Scr1, a transcription factor involved in glucose repression, localized to the nucleus under glucose-rich conditions and to the cytoplasm during glucose starvation in wild-type cells. In contrast, in the ssp2A mutant, Scr1 localized to the nucleus in cells grown in glucose-rich medium as well as in glucose-starved cells. Immunoblot analysis showed that Ssp2 is required for the phosphorylation of Scr1 upon glucose deprivation. Mutation of five putative Ssp2 recognition sites in Scr1 prevented glucose derepression of invertase in glucose-starved cells. These results indicate that Ssp2 regulates phosphorylation and subcellular localization of Scr1 in response to glucose.

AB - The function of two fission yeast genes, SPCC74.03c/ssp2 + and SPAC23H4.02/ppk9 +, encoding an Snf1-like protein kinase were investigated. Deletion of ssp2 + caused a partial defect in glucose derepression of inv1 +,fbp1 +, and gld1 + and in assimilation of sucrose and glycerol, while a mutation in ppk9 + had no apparent effect. Scr1, a transcription factor involved in glucose repression, localized to the nucleus under glucose-rich conditions and to the cytoplasm during glucose starvation in wild-type cells. In contrast, in the ssp2A mutant, Scr1 localized to the nucleus in cells grown in glucose-rich medium as well as in glucose-starved cells. Immunoblot analysis showed that Ssp2 is required for the phosphorylation of Scr1 upon glucose deprivation. Mutation of five putative Ssp2 recognition sites in Scr1 prevented glucose derepression of invertase in glucose-starved cells. These results indicate that Ssp2 regulates phosphorylation and subcellular localization of Scr1 in response to glucose.

UR - http://www.scopus.com/inward/record.url?scp=84856688493&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84856688493&partnerID=8YFLogxK

U2 - 10.1128/EC.05268-11

DO - 10.1128/EC.05268-11

M3 - Article

C2 - 22140232

AN - SCOPUS:84856688493

VL - 11

SP - 159

EP - 167

JO - Eukaryotic Cell

JF - Eukaryotic Cell

SN - 1535-9778

IS - 2

ER -