Snx3 regulates recycling of the transferrin receptor and iron assimilation

Caiyong Chen, Daniel Garcia-Santos, Yuichi Ishikawa, Alexandra Seguin, Liangtao Li, Katherine H. Fegan, Gordon J. Hildick-Smith, Dhvanit I. Shah, Jeffrey D. Cooney, Wen Chen, Matthew J. King, Yvette Y. Yien, Iman J. Schultz, Heidi Anderson, Arthur J. Dalton, Matthew L. Freedman, Paul D. Kingsley, James Palis, Shilpa M. Hattangadi, Harvey F. LodishDiane M. Ward, Jerry Kaplan, Takahiro Maeda, Prem Ponka, Barry H. Paw

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Sorting of endocytic ligands and receptors is critical for diverse cellular processes. The physiological significance of endosomal sorting proteins in vertebrates, however, remains largely unknown. Here we report that sorting nexin 3 (Snx3) facilitates the recycling of transferrin receptor (Tfrc) and thus is required for the proper delivery of iron to erythroid progenitors. Snx3 is highly expressed in vertebrate hematopoietic tissues. Silencing of Snx3 results in anemia and hemoglobin defects in vertebrates due to impaired transferrin (Tf)-mediated iron uptake and its accumulation in early endosomes. This impaired iron assimilation can be complemented with non-Tf iron chelates. We show that Snx3 and Vps35, a component of the retromer, interact with Tfrc to sort it to the recycling endosomes. Our findings uncover a role of Snx3 in regulating Tfrc recycling, iron homeostasis, and erythropoiesis. Thus, the identification of Snx3 provides a genetic tool for exploring erythropoiesis and disorders of iron metabolism.

Original languageEnglish
Pages (from-to)343-352
Number of pages10
JournalCell metabolism
Volume17
Issue number3
DOIs
Publication statusPublished - Mar 5 2013
Externally publishedYes

Fingerprint

Sorting Nexins
Transferrin Receptors
Recycling
Iron
Vertebrates
Erythropoiesis
Endosomes
Iron Metabolism Disorders
Iron Chelating Agents
Protein Transport
Transferrin
Anemia
Hemoglobins
Homeostasis
Ligands

All Science Journal Classification (ASJC) codes

  • Physiology
  • Molecular Biology
  • Cell Biology

Cite this

Chen, C., Garcia-Santos, D., Ishikawa, Y., Seguin, A., Li, L., Fegan, K. H., ... Paw, B. H. (2013). Snx3 regulates recycling of the transferrin receptor and iron assimilation. Cell metabolism, 17(3), 343-352. https://doi.org/10.1016/j.cmet.2013.01.013

Snx3 regulates recycling of the transferrin receptor and iron assimilation. / Chen, Caiyong; Garcia-Santos, Daniel; Ishikawa, Yuichi; Seguin, Alexandra; Li, Liangtao; Fegan, Katherine H.; Hildick-Smith, Gordon J.; Shah, Dhvanit I.; Cooney, Jeffrey D.; Chen, Wen; King, Matthew J.; Yien, Yvette Y.; Schultz, Iman J.; Anderson, Heidi; Dalton, Arthur J.; Freedman, Matthew L.; Kingsley, Paul D.; Palis, James; Hattangadi, Shilpa M.; Lodish, Harvey F.; Ward, Diane M.; Kaplan, Jerry; Maeda, Takahiro; Ponka, Prem; Paw, Barry H.

In: Cell metabolism, Vol. 17, No. 3, 05.03.2013, p. 343-352.

Research output: Contribution to journalArticle

Chen, C, Garcia-Santos, D, Ishikawa, Y, Seguin, A, Li, L, Fegan, KH, Hildick-Smith, GJ, Shah, DI, Cooney, JD, Chen, W, King, MJ, Yien, YY, Schultz, IJ, Anderson, H, Dalton, AJ, Freedman, ML, Kingsley, PD, Palis, J, Hattangadi, SM, Lodish, HF, Ward, DM, Kaplan, J, Maeda, T, Ponka, P & Paw, BH 2013, 'Snx3 regulates recycling of the transferrin receptor and iron assimilation', Cell metabolism, vol. 17, no. 3, pp. 343-352. https://doi.org/10.1016/j.cmet.2013.01.013
Chen C, Garcia-Santos D, Ishikawa Y, Seguin A, Li L, Fegan KH et al. Snx3 regulates recycling of the transferrin receptor and iron assimilation. Cell metabolism. 2013 Mar 5;17(3):343-352. https://doi.org/10.1016/j.cmet.2013.01.013
Chen, Caiyong ; Garcia-Santos, Daniel ; Ishikawa, Yuichi ; Seguin, Alexandra ; Li, Liangtao ; Fegan, Katherine H. ; Hildick-Smith, Gordon J. ; Shah, Dhvanit I. ; Cooney, Jeffrey D. ; Chen, Wen ; King, Matthew J. ; Yien, Yvette Y. ; Schultz, Iman J. ; Anderson, Heidi ; Dalton, Arthur J. ; Freedman, Matthew L. ; Kingsley, Paul D. ; Palis, James ; Hattangadi, Shilpa M. ; Lodish, Harvey F. ; Ward, Diane M. ; Kaplan, Jerry ; Maeda, Takahiro ; Ponka, Prem ; Paw, Barry H. / Snx3 regulates recycling of the transferrin receptor and iron assimilation. In: Cell metabolism. 2013 ; Vol. 17, No. 3. pp. 343-352.
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