Soaking RNAi in Bombyx mori BmN4-SID1 cells arrests cell cycle progression

Hiroaki Mon, Zhiqing Li, Isao Kobayashi, Shuichiro Tomita, Jae Man Lee, Hideki Sezutsu, Toshiki Tamura, Takahiro Kusakabe

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

RNA interference (RNAi) is an evolutionarily conserved mechanism for sequence-specific gene silencing. Previously, the BmN4-SID1 cell expressing Caenorhabditis ele gans SID-1 was established, in which soaking RNAi could induce effective gene silencing. To establish its utility, 6 cell cycle progression related cDNAs, CDK1, MYC, MYB, RNRS, CDT1, and GEMININ, were isolated from the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae), and their expressions were further silenced by soaking RNAi in the BmN4-SID1 cells. The cell cycle progression analysis using flow cytometer demonstrated that the small amount of double stranded RNA was enough to arrest cell cycle progression at the specific cell phases. These data suggest that RNAi in the BmN4-SID1 cells can be used as a powerful tool for loss-of-function analysis of B. mori genes.

Original languageEnglish
Article number155
JournalJournal of Insect Science
Volume13
DOIs
Publication statusPublished - Dec 2013

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Bombyx mori
RNA interference
soaking
gene silencing
cell cycle
Caenorhabditis
Bombycidae
cells
double-stranded RNA
silkworms
Lepidoptera
cell cycle checkpoints
genes

All Science Journal Classification (ASJC) codes

  • Insect Science

Cite this

Soaking RNAi in Bombyx mori BmN4-SID1 cells arrests cell cycle progression. / Mon, Hiroaki; Li, Zhiqing; Kobayashi, Isao; Tomita, Shuichiro; Lee, Jae Man; Sezutsu, Hideki; Tamura, Toshiki; Kusakabe, Takahiro.

In: Journal of Insect Science, Vol. 13, 155, 12.2013.

Research output: Contribution to journalArticle

Mon, Hiroaki ; Li, Zhiqing ; Kobayashi, Isao ; Tomita, Shuichiro ; Lee, Jae Man ; Sezutsu, Hideki ; Tamura, Toshiki ; Kusakabe, Takahiro. / Soaking RNAi in Bombyx mori BmN4-SID1 cells arrests cell cycle progression. In: Journal of Insect Science. 2013 ; Vol. 13.
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