Soaking RNAi-mediated modification of Sf9 cells for baculovirus expression system by ectopic expression of Caenorhabditis elegans SID-1

Jian Xu, Yudai Nagata, Hiroaki Mon, Zhiqing Li, Li Zhu, Kazuhiro Iiyama, Takahiro Kusakabe, Man Lee

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

RNA interference (RNAi) is a biological phenomenon that silences the expression of genes of interest. Passive double-stranded RNA (dsRNA) uptake has been uniquely observed in Caenorhabditis elegans due to the expression of systemic RNAi defective-1 (SID-1). We report that ectopic expression of CeSID-1 endows the Sf9 cells with a capacity for soaking RNAi. Soaking the Sf9-SID1 with dsRNA corresponding to either exogenous or endogenous target genes induced a significant decrease in the amount of mRNA or protein. These results enabled us to modify the target proteins of baculovirus expression vector system in both quantities and posttranslational modifications. The current low-cost and high-efficiency RNAi system is useful for high-throughput gene function analysis and mass production of recombinant protein.

Original languageEnglish
Pages (from-to)5921-5931
Number of pages11
JournalApplied Microbiology and Biotechnology
Volume97
Issue number13
DOIs
Publication statusPublished - Jul 1 2013

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Sf9 Cells
Baculoviridae
Caenorhabditis elegans
RNA Interference
Double-Stranded RNA
Biological Phenomena
Post Translational Protein Processing
Recombinant Proteins
Genes
Proteins
Gene Expression
Costs and Cost Analysis
Messenger RNA
Ectopic Gene Expression

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Soaking RNAi-mediated modification of Sf9 cells for baculovirus expression system by ectopic expression of Caenorhabditis elegans SID-1. / Xu, Jian; Nagata, Yudai; Mon, Hiroaki; Li, Zhiqing; Zhu, Li; Iiyama, Kazuhiro; Kusakabe, Takahiro; Lee, Man.

In: Applied Microbiology and Biotechnology, Vol. 97, No. 13, 01.07.2013, p. 5921-5931.

Research output: Contribution to journalArticle

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