Sodium tauroursodeoxycholate prevents paraquat-induced cell death by suppressing endoplasmic reticulum stress responses in human lung epithelial A549 cells

Tomohiro Omura, Masaru Asari, Joe Yamamoto, Kumiko Oka, Chisato Hoshina, Chikatoshi Maseda, Toshio Awaya, Yoshikazu Tasaki, Hiroshi Shiono, Atsushi Yonezawa, Satohiro Masuda, Kazuo Matsubara, Keiko Shimizu

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26 Citations (Scopus)

Abstract

Paraquat is a commonly used herbicide; however, it is highly toxic to humans and animals. Exposure to paraquat causes severe lung damage, leading to pulmonary fibrosis. However, it has not been well clarified as how paraquat causes cellular damage, and there is no established standard therapy for paraquat poisoning. Meanwhile, endoplasmic reticulum stress (ERS) is reported to be one of the causative factors in many diseases, although mammalian cells have a defense mechanism against ERS-induced apoptosis (unfolded protein response). Here, we demonstrated that paraquat changed the expression levels of unfolded protein response-related molecules, resulting in ERS-related cell death in human lung epithelial A549 cells. Moreover, treatment with sodium tauroursodeoxycholate (TUDCA), a chemical chaperone, crucially rescued cells from death caused by exposure to paraquat. These results indicate that paraquat toxicity may be associated with ERS-related molecules/events. Through chemical chaperone activity, treatment with TUDCA reduced paraquat-induced ERS and mildly suppressed cell death. Our findings also suggest that TUDCA treatment represses the onset of pulmonary fibrosis caused by paraquat, and therefore chemical chaperones may have novel therapeutic potential for the treatment of paraquat poisoning.

Original languageEnglish
Pages (from-to)689-694
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume432
Issue number4
DOIs
Publication statusPublished - Mar 22 2013

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Omura, T., Asari, M., Yamamoto, J., Oka, K., Hoshina, C., Maseda, C., Awaya, T., Tasaki, Y., Shiono, H., Yonezawa, A., Masuda, S., Matsubara, K., & Shimizu, K. (2013). Sodium tauroursodeoxycholate prevents paraquat-induced cell death by suppressing endoplasmic reticulum stress responses in human lung epithelial A549 cells. Biochemical and Biophysical Research Communications, 432(4), 689-694. https://doi.org/10.1016/j.bbrc.2013.01.131