Solid-phase peptide synthesis in a microfluidic device

Tatsuo Maruyama; Jun Ichi Uchida; Tomohiro Ohkawa; Fukiko Kubota; Noriho Kamiya; Masahiro Goto

doi:10.1252/kakoronbunshu.30.180

Solid-phase peptide synthesis in a microfluidic device

Tatsuo Maruyama, Jun Ichi Uchida, Tomohiro Ohkawa, Fukiko Kubota, Noriho Kamiya, Masahiro Goto

Applied Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

The solid-phase synthesis of oligopeptide was achieved for the first time in a microfluidic device. We fabricated a microchannel (width 300 μm, depth 160 μm) with a bead trap on a glass plate. Resin beads were loaded in the bead trap for the solid-phase peptide synthesis, and the synthesis of pentapeptide (Met-enkephalin) was conducted in the microchannel. The yield of the synthesized pentapeptide was confirmed to be 20% by MALDI-TOF MS and HPLC analyses.

Original language	English
Pages (from-to)	180-182
Number of pages	3
Journal	kagaku kogaku ronbunshu
Volume	30
Issue number	2
DOIs	https://doi.org/10.1252/kakoronbunshu.30.180
Publication status	Published - Mar 2004

All Science Journal Classification (ASJC) codes

Chemistry(all)
Chemical Engineering(all)

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AU - Maruyama, Tatsuo

AU - Uchida, Jun Ichi

AU - Ohkawa, Tomohiro

AU - Kubota, Fukiko

AU - Kamiya, Noriho

AU - Goto, Masahiro

PY - 2004/3

Y1 - 2004/3

N2 - The solid-phase synthesis of oligopeptide was achieved for the first time in a microfluidic device. We fabricated a microchannel (width 300 μm, depth 160 μm) with a bead trap on a glass plate. Resin beads were loaded in the bead trap for the solid-phase peptide synthesis, and the synthesis of pentapeptide (Met-enkephalin) was conducted in the microchannel. The yield of the synthesized pentapeptide was confirmed to be 20% by MALDI-TOF MS and HPLC analyses.

AB - The solid-phase synthesis of oligopeptide was achieved for the first time in a microfluidic device. We fabricated a microchannel (width 300 μm, depth 160 μm) with a bead trap on a glass plate. Resin beads were loaded in the bead trap for the solid-phase peptide synthesis, and the synthesis of pentapeptide (Met-enkephalin) was conducted in the microchannel. The yield of the synthesized pentapeptide was confirmed to be 20% by MALDI-TOF MS and HPLC analyses.

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JO - Kagaku Kogaku Ronbunshu

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Solid-phase peptide synthesis in a microfluidic device

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