Solid-phase peptide synthesis in a microfluidic device

Tatsuo Maruyama, Jun Ichi Uchida, Tomohiro Ohkawa, Fukiko Kubota, Noriho Kamiya, Masahiro Goto

Research output: Contribution to journalArticlepeer-review

Abstract

The solid-phase synthesis of oligopeptide was achieved for the first time in a microfluidic device. We fabricated a microchannel (width 300 μm, depth 160 μm) with a bead trap on a glass plate. Resin beads were loaded in the bead trap for the solid-phase peptide synthesis, and the synthesis of pentapeptide (Met-enkephalin) was conducted in the microchannel. The yield of the synthesized pentapeptide was confirmed to be 20% by MALDI-TOF MS and HPLC analyses.

Original languageEnglish
Pages (from-to)180-182
Number of pages3
Journalkagaku kogaku ronbunshu
Volume30
Issue number2
DOIs
Publication statusPublished - Mar 1 2004

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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