This lecture reports on the protein transfer from an aqueous phase into ionic liquids (ILs) with dicyclohexano-18-crown-6 (DCH18C6), and the functional change of cytochrome c (Cyt-c) dissolved in ILs. Further, a protein is modified with comb-shaped poly (ethylene glycol), PM13. PM13 modified subtilisin (PM13-Sub) could be solubilized clearly in a wide range of pure ILs. This markedly high activity in the IL was superior to that in organic solvents commonly used for enzymatic catalysis. PM13-Sub was also found to retain its catalytic activity in the ILs for a prolonged period.
|Number of pages||2|
|Publication status||Published - Dec 1 2006|
|Event||55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan|
Duration: Sep 20 2006 → Sep 22 2006
|Other||55th Society of Polymer Science Japan Symposium on Macromolecules|
|Period||9/20/06 → 9/22/06|
All Science Journal Classification (ASJC) codes