Solubilization and high activity of enzymes in ionic liquids by modification with comb-shaped poly (ethylene glycol)

Kazunori Nakashima; Tatsuo Maruyama; Noriho Kamiya; Masahiro Goto

Solubilization and high activity of enzymes in ionic liquids by modification with comb-shaped poly (ethylene glycol)

Kazunori Nakashima, Tatsuo Maruyama, Noriho Kamiya, Masahiro Goto

Applied Chemistry

Research output: Contribution to conference › Paper › peer-review

Abstract

Subtilisin Carlsberg was modified with comb-shaped poly (ethylene glycol), PM₁₃. PM₁₃ modified subtilisin (PM13-Sub) could be solubilized clearly in a wide range of pure ILs, whereas native subtilisin was not soluble in any ILs. Furthermore, PM13-Sub dissolved in ILs exhibited the excellent catalytic activity in transesterification of N-acetyl-L-phenylalanine ethyl ester with 1-butanol. This markedly high activity in the IL was superior to that in organic solvents commonly used for enzymatic catalysis. PM13-Sub was also found to retain its catalytic activity in the ILs for a prolonged period.

Original language	English
Pages	4647-4648
Number of pages	2
Publication status	Published - 2005
Event	54th SPSJ Symposium on Macromolecules - Yamagata, Japan Duration: Sept 20 2005 → Sept 22 2005

Other

Other	54th SPSJ Symposium on Macromolecules
Country/Territory	Japan
City	Yamagata
Period	9/20/05 → 9/22/05

All Science Journal Classification (ASJC) codes

Engineering(all)

Cite this

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title = "Solubilization and high activity of enzymes in ionic liquids by modification with comb-shaped poly (ethylene glycol)",

abstract = "Subtilisin Carlsberg was modified with comb-shaped poly (ethylene glycol), PM13. PM13 modified subtilisin (PM13-Sub) could be solubilized clearly in a wide range of pure ILs, whereas native subtilisin was not soluble in any ILs. Furthermore, PM13-Sub dissolved in ILs exhibited the excellent catalytic activity in transesterification of N-acetyl-L-phenylalanine ethyl ester with 1-butanol. This markedly high activity in the IL was superior to that in organic solvents commonly used for enzymatic catalysis. PM13-Sub was also found to retain its catalytic activity in the ILs for a prolonged period.",

author = "Kazunori Nakashima and Tatsuo Maruyama and Noriho Kamiya and Masahiro Goto",

year = "2005",

language = "English",

pages = "4647--4648",

note = "54th SPSJ Symposium on Macromolecules ; Conference date: 20-09-2005 Through 22-09-2005",

}

TY - CONF

T1 - Solubilization and high activity of enzymes in ionic liquids by modification with comb-shaped poly (ethylene glycol)

AU - Nakashima, Kazunori

AU - Maruyama, Tatsuo

AU - Kamiya, Noriho

AU - Goto, Masahiro

PY - 2005

Y1 - 2005

N2 - Subtilisin Carlsberg was modified with comb-shaped poly (ethylene glycol), PM13. PM13 modified subtilisin (PM13-Sub) could be solubilized clearly in a wide range of pure ILs, whereas native subtilisin was not soluble in any ILs. Furthermore, PM13-Sub dissolved in ILs exhibited the excellent catalytic activity in transesterification of N-acetyl-L-phenylalanine ethyl ester with 1-butanol. This markedly high activity in the IL was superior to that in organic solvents commonly used for enzymatic catalysis. PM13-Sub was also found to retain its catalytic activity in the ILs for a prolonged period.

AB - Subtilisin Carlsberg was modified with comb-shaped poly (ethylene glycol), PM13. PM13 modified subtilisin (PM13-Sub) could be solubilized clearly in a wide range of pure ILs, whereas native subtilisin was not soluble in any ILs. Furthermore, PM13-Sub dissolved in ILs exhibited the excellent catalytic activity in transesterification of N-acetyl-L-phenylalanine ethyl ester with 1-butanol. This markedly high activity in the IL was superior to that in organic solvents commonly used for enzymatic catalysis. PM13-Sub was also found to retain its catalytic activity in the ILs for a prolonged period.

UR - http://www.scopus.com/inward/record.url?scp=33645559548&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645559548&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33645559548

SP - 4647

EP - 4648

T2 - 54th SPSJ Symposium on Macromolecules

Y2 - 20 September 2005 through 22 September 2005

ER -

Solubilization and high activity of enzymes in ionic liquids by modification with comb-shaped poly (ethylene glycol)

Abstract

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