Soluble branched β-(1,4)glucans from Acetobacter species show strong activities to induce interleukin-12 in vitro and inhibit T-helper 2 cellular response with immunoglobulin E production in vivo

Kimika Saito, Toshiki Yajima, Hitoshi Nishimura, Keiko Aiba, Ryotaro Ishimitsu, Tetsuya Matsuguchi, Takashi Fushimi, Yoshifumi Ohshima, Yoshinori Tsukamoto, Yasunobu Yoshikai

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    36 Citations (Scopus)

    Abstract

    An extracellular polysaccharide, AC-1, produced by Acetobacter polysaccharogenes is composed of β-(1,4)glucan with branches of glucosyl residues. We found that AC-1 showed a strong activity to induce production of interleukin-12 p40 and tumor necrosis factor-α by macrophage cell lines in vitro. Cellulase treatment completely abolished the activity of AC-1 to induce tumor necrosis factor-α production by macrophages, whereas treatment of AC-1 with polymyxin B or proteinase did not affect the activity. Results of experiments using Toll-like receptor (TLR) 4-deficient mice and TLR4-transfected human cell line indicated that TLR4 is involved in pattern recognition of AC-1. In vivo administration of AC-1 significantly reduced the serum levels of ovalbumin (OVA)-specific IgE and interleukin-4 production by T cells in response to OVA in mice immunized with OVA. AC-1, a soluble branched β-(1,4)glucan may be useful in prevention and treatment of allergic disorders with IgE production.

    Original languageEnglish
    Pages (from-to)38571-38578
    Number of pages8
    JournalJournal of Biological Chemistry
    Volume278
    Issue number40
    DOIs
    Publication statusPublished - Oct 3 2003

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Molecular Biology
    • Cell Biology

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