TY - JOUR
T1 - Soluble branched β-(1,4)glucans from Acetobacter species show strong activities to induce interleukin-12 in vitro and inhibit T-helper 2 cellular response with immunoglobulin E production in vivo
AU - Saito, Kimika
AU - Yajima, Toshiki
AU - Nishimura, Hitoshi
AU - Aiba, Keiko
AU - Ishimitsu, Ryotaro
AU - Matsuguchi, Tetsuya
AU - Fushimi, Takashi
AU - Ohshima, Yoshifumi
AU - Tsukamoto, Yoshinori
AU - Yoshikai, Yasunobu
PY - 2003/10/3
Y1 - 2003/10/3
N2 - An extracellular polysaccharide, AC-1, produced by Acetobacter polysaccharogenes is composed of β-(1,4)glucan with branches of glucosyl residues. We found that AC-1 showed a strong activity to induce production of interleukin-12 p40 and tumor necrosis factor-α by macrophage cell lines in vitro. Cellulase treatment completely abolished the activity of AC-1 to induce tumor necrosis factor-α production by macrophages, whereas treatment of AC-1 with polymyxin B or proteinase did not affect the activity. Results of experiments using Toll-like receptor (TLR) 4-deficient mice and TLR4-transfected human cell line indicated that TLR4 is involved in pattern recognition of AC-1. In vivo administration of AC-1 significantly reduced the serum levels of ovalbumin (OVA)-specific IgE and interleukin-4 production by T cells in response to OVA in mice immunized with OVA. AC-1, a soluble branched β-(1,4)glucan may be useful in prevention and treatment of allergic disorders with IgE production.
AB - An extracellular polysaccharide, AC-1, produced by Acetobacter polysaccharogenes is composed of β-(1,4)glucan with branches of glucosyl residues. We found that AC-1 showed a strong activity to induce production of interleukin-12 p40 and tumor necrosis factor-α by macrophage cell lines in vitro. Cellulase treatment completely abolished the activity of AC-1 to induce tumor necrosis factor-α production by macrophages, whereas treatment of AC-1 with polymyxin B or proteinase did not affect the activity. Results of experiments using Toll-like receptor (TLR) 4-deficient mice and TLR4-transfected human cell line indicated that TLR4 is involved in pattern recognition of AC-1. In vivo administration of AC-1 significantly reduced the serum levels of ovalbumin (OVA)-specific IgE and interleukin-4 production by T cells in response to OVA in mice immunized with OVA. AC-1, a soluble branched β-(1,4)glucan may be useful in prevention and treatment of allergic disorders with IgE production.
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U2 - 10.1074/jbc.M304948200
DO - 10.1074/jbc.M304948200
M3 - Article
C2 - 12799362
AN - SCOPUS:0141755307
VL - 278
SP - 38571
EP - 38578
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 40
ER -