Solution structure of human insulin-like growth factor II; Recognition sites for receptors and binding proteins

Hiroaki Terasawa, Daisuke Kohda, Hideki Hatanaka, Koji Nagata, Nobuyuki Higashihashi, Hiroyuki Fujiwara, Katsu Ichi Sakano, Fuyuhiko Inagaki

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The three-dimensional structure of human insulin-like growth factor II was determined at high resolution in aqueous solution by NMR and simulated annealing based calculations. The structure is quite similar to those of insulin and insulin-like growth factor I, which consists of an α-helix followed by a turn and a strand in the B-region and two antiparallel α-helices in the A-region. However, the regions of Ala1-Glu6, Pro31-Arg40 and Thr62-Glu67 are not well-defined for lack of distance constraints, possibly due to motional flexibility. Based on the resultant structure and the results of structure-activity relationships, we propose the interaction sites of insulin-like growth factor II with the type 2 insulin-like growth factor receptor and the insulin-like growth factor binding proteins. These sites partially overlap with each other at the opposite side of the putative binding surface to the insulin receptor and the type 1 insulin-like growth factor receptor. We also discuss the interaction modes of insulin-like growth factor II with the insulin receptor and the type 1 insulin-like growth factor receptor.

Original languageEnglish
Pages (from-to)5590-5597
Number of pages8
JournalEMBO Journal
Volume13
Issue number23
Publication statusPublished - Dec 1 1994
Externally publishedYes

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Insulin-Like Growth Factor II
Carrier Proteins
IGF Type 1 Receptor
Insulin Receptor
IGF Type 2 Receptor
Insulin-Like Growth Factor Binding Proteins
Structure-Activity Relationship
Simulated annealing
Insulin-Like Growth Factor I
Nuclear magnetic resonance
Insulin

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Terasawa, H., Kohda, D., Hatanaka, H., Nagata, K., Higashihashi, N., Fujiwara, H., ... Inagaki, F. (1994). Solution structure of human insulin-like growth factor II; Recognition sites for receptors and binding proteins. EMBO Journal, 13(23), 5590-5597.

Solution structure of human insulin-like growth factor II; Recognition sites for receptors and binding proteins. / Terasawa, Hiroaki; Kohda, Daisuke; Hatanaka, Hideki; Nagata, Koji; Higashihashi, Nobuyuki; Fujiwara, Hiroyuki; Sakano, Katsu Ichi; Inagaki, Fuyuhiko.

In: EMBO Journal, Vol. 13, No. 23, 01.12.1994, p. 5590-5597.

Research output: Contribution to journalArticle

Terasawa, H, Kohda, D, Hatanaka, H, Nagata, K, Higashihashi, N, Fujiwara, H, Sakano, KI & Inagaki, F 1994, 'Solution structure of human insulin-like growth factor II; Recognition sites for receptors and binding proteins', EMBO Journal, vol. 13, no. 23, pp. 5590-5597.
Terasawa H, Kohda D, Hatanaka H, Nagata K, Higashihashi N, Fujiwara H et al. Solution structure of human insulin-like growth factor II; Recognition sites for receptors and binding proteins. EMBO Journal. 1994 Dec 1;13(23):5590-5597.
Terasawa, Hiroaki ; Kohda, Daisuke ; Hatanaka, Hideki ; Nagata, Koji ; Higashihashi, Nobuyuki ; Fujiwara, Hiroyuki ; Sakano, Katsu Ichi ; Inagaki, Fuyuhiko. / Solution structure of human insulin-like growth factor II; Recognition sites for receptors and binding proteins. In: EMBO Journal. 1994 ; Vol. 13, No. 23. pp. 5590-5597.
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