Somatic DNA recombination yielding circular DNA and deletion of a genomic region in embryonic brain

Toyoki Maeda, Yoshiharu Chijiiwa, Hideo Tsuji, Saburo Sakoda, Kenzaburo Tani, Tomokazu Suzuki

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

In this study, a mouse genomic region is identified that undergoes DNA rearrangement and yields circular DNA in brain during embryogenesis. External region-directed inverse polymerase chain reaction on circular DNA extracted from late embryonic brain tissue repeatedly detected DNA of this region containing recombination joints. Wide-range genomic PCR and digestion-circularization PCR analysis showed this region underwent recombination accompanied with deletion of intervening sequences, including the circularized regions. This region was mapped by fluorescence in situ hybridization to C1 on mouse chromosome 16, where no gene and no physiological DNA rearrangement had been identified. DNA sequence in the region has segmental homology to an orthologous region on human chromosome 3q.13. These observations demonstrated somatic DNA recombination yielding genomic deletions in brain during embryogenesis.

Original languageEnglish
Pages (from-to)1117-1123
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume319
Issue number4
DOIs
Publication statusPublished - Jul 9 2004

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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