Somatic DNA recombination yielding circular DNA and deletion of a genomic region in embryonic brain

Toyoki Maeda, Yoshiharu Chijiiwa, Hideo Tsuji, Saburo Sakoda, Kenzaburo Tani, Tomokazu Suzuki

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In this study, a mouse genomic region is identified that undergoes DNA rearrangement and yields circular DNA in brain during embryogenesis. External region-directed inverse polymerase chain reaction on circular DNA extracted from late embryonic brain tissue repeatedly detected DNA of this region containing recombination joints. Wide-range genomic PCR and digestion-circularization PCR analysis showed this region underwent recombination accompanied with deletion of intervening sequences, including the circularized regions. This region was mapped by fluorescence in situ hybridization to C1 on mouse chromosome 16, where no gene and no physiological DNA rearrangement had been identified. DNA sequence in the region has segmental homology to an orthologous region on human chromosome 3q.13. These observations demonstrated somatic DNA recombination yielding genomic deletions in brain during embryogenesis.

Original languageEnglish
Pages (from-to)1117-1123
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume319
Issue number4
DOIs
Publication statusPublished - Jul 9 2004

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Circular DNA
Genetic Recombination
Brain
Gene Rearrangement
Polymerase Chain Reaction
Embryonic Development
DNA
Chromosomes
Chromosomes, Human, Pair 16
Recombinant DNA
Human Chromosomes
Fluorescence In Situ Hybridization
Introns
Digestion
Polymerase chain reaction
DNA sequences
Genes
Fluorescence
Tissue

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Somatic DNA recombination yielding circular DNA and deletion of a genomic region in embryonic brain. / Maeda, Toyoki; Chijiiwa, Yoshiharu; Tsuji, Hideo; Sakoda, Saburo; Tani, Kenzaburo; Suzuki, Tomokazu.

In: Biochemical and Biophysical Research Communications, Vol. 319, No. 4, 09.07.2004, p. 1117-1123.

Research output: Contribution to journalArticle

Maeda, Toyoki ; Chijiiwa, Yoshiharu ; Tsuji, Hideo ; Sakoda, Saburo ; Tani, Kenzaburo ; Suzuki, Tomokazu. / Somatic DNA recombination yielding circular DNA and deletion of a genomic region in embryonic brain. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 319, No. 4. pp. 1117-1123.
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