In Cyclamen persicum 'Anneke', explants from the various vegetative organs of aseptic seedling formed embryoids. The optimal responses were recorded in Murashige and Skoog (MS) medium enriched with 5.0 μM 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5 μM kinetin and 3-6% sucrose. Embryogenesis was enhanced at higher temperature of 25-30°C. On the other hand, light inhibited embryogenesis. Histological and morphological studies confirmed that the embryoids were indeed somatic embryos.
All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science
- Plant Science