Somatic embryogenesis of Cyclamen persicum Mill. 'Anneke' from aseptic seedlings

Takejiro Takamura; Ikuo Miyajima; Eisuke Matsuo

doi:10.1007/BF01690246

Somatic embryogenesis of Cyclamen persicum Mill. 'Anneke' from aseptic seedlings

Takejiro Takamura, Ikuo Miyajima, Eisuke Matsuo

Institute of Tropical Agriculture

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

In Cyclamen persicum 'Anneke', explants from the various vegetative organs of aseptic seedling formed embryoids. The optimal responses were recorded in Murashige and Skoog (MS) medium enriched with 5.0 μM 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5 μM kinetin and 3-6% sucrose. Embryogenesis was enhanced at higher temperature of 25-30°C. On the other hand, light inhibited embryogenesis. Histological and morphological studies confirmed that the embryoids were indeed somatic embryos.

Original language	English
Pages (from-to)	22-25
Number of pages	4
Journal	Plant Cell Reports
Volume	15
Issue number	1-2
DOIs	https://doi.org/10.1007/BF01690246
Publication status	Published - Jan 1 1995

All Science Journal Classification (ASJC) codes

Agronomy and Crop Science
Plant Science

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10.1007/BF01690246

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abstract = "In Cyclamen persicum 'Anneke', explants from the various vegetative organs of aseptic seedling formed embryoids. The optimal responses were recorded in Murashige and Skoog (MS) medium enriched with 5.0 μM 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5 μM kinetin and 3-6% sucrose. Embryogenesis was enhanced at higher temperature of 25-30°C. On the other hand, light inhibited embryogenesis. Histological and morphological studies confirmed that the embryoids were indeed somatic embryos.",

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AU - Miyajima, Ikuo

AU - Matsuo, Eisuke

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N2 - In Cyclamen persicum 'Anneke', explants from the various vegetative organs of aseptic seedling formed embryoids. The optimal responses were recorded in Murashige and Skoog (MS) medium enriched with 5.0 μM 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5 μM kinetin and 3-6% sucrose. Embryogenesis was enhanced at higher temperature of 25-30°C. On the other hand, light inhibited embryogenesis. Histological and morphological studies confirmed that the embryoids were indeed somatic embryos.

AB - In Cyclamen persicum 'Anneke', explants from the various vegetative organs of aseptic seedling formed embryoids. The optimal responses were recorded in Murashige and Skoog (MS) medium enriched with 5.0 μM 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5 μM kinetin and 3-6% sucrose. Embryogenesis was enhanced at higher temperature of 25-30°C. On the other hand, light inhibited embryogenesis. Histological and morphological studies confirmed that the embryoids were indeed somatic embryos.

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Somatic embryogenesis of Cyclamen persicum Mill. 'Anneke' from aseptic seedlings

Abstract

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