Some properties and occurrence of cytochrome c-552 in the aerobic photosynthetic bacterium Roseobacter denitrificans

Ken ichiro Takamiya, Yuzo Shioi, Masakazu Morita, Hiroyuki Arata, Minoru Shimizu, Michio Doi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Characteristics and occurrence of cytochrome c-552 from an aerobic photosynthetic bacterium, Roseobacter denitrificans, were described. Relative molecular mass of the cytrochrome was 13.5 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and 15,000 by gel filtration. This cytochrome was a acidic protein having a pI of 5.6 and Em was +215 mV at pH 7.0. Absorption peaks were at 278, 408 and 524 nm in the oxidized form and 416, 523 and 552 nm in the reduced form. Amino acid composition and N-terminal amino acid sequence of cytochrome c-552 determined for 24 residues had low similarities to those of cytochrome c-551 of this bacterium, which is homologous to cytochrome c2, although the physico-chemical properties of these two cytochromes were similar to each other. Cytochrome c-552 was maximally synthesized in the light under aerobic conditions but not in the dark. The synthesis also occurred in the presence of alternative acceptors such as trimethylamine N-oxide (TMAO) and nitrate under anaerobic conditions. Our results suggest that cytochrome c-552 is involved in TMAO respiration and denitrification in R. denitrificans, although the effect of light remains to be solved.

Original languageEnglish
Pages (from-to)51-56
Number of pages6
JournalArchives of Microbiology
Volume159
Issue number1
DOIs
Publication statusPublished - Jan 1 1993

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Roseobacter
Aerobic Bacteria
Cytochromes c
Bacteria
Cytochromes
Cytochromes c2
Light
Amino Acids
Denitrification
Molecular mass
Electrophoresis
Sodium Dodecyl Sulfate
Nitrates
Chemical properties
Gel Chromatography
Polyacrylamide Gel Electrophoresis
Amino Acid Sequence
Respiration
Gels
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Some properties and occurrence of cytochrome c-552 in the aerobic photosynthetic bacterium Roseobacter denitrificans. / Takamiya, Ken ichiro; Shioi, Yuzo; Morita, Masakazu; Arata, Hiroyuki; Shimizu, Minoru; Doi, Michio.

In: Archives of Microbiology, Vol. 159, No. 1, 01.01.1993, p. 51-56.

Research output: Contribution to journalArticle

Takamiya, Ken ichiro ; Shioi, Yuzo ; Morita, Masakazu ; Arata, Hiroyuki ; Shimizu, Minoru ; Doi, Michio. / Some properties and occurrence of cytochrome c-552 in the aerobic photosynthetic bacterium Roseobacter denitrificans. In: Archives of Microbiology. 1993 ; Vol. 159, No. 1. pp. 51-56.
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