Sorting nexin homologues are targets of phosphatidylinositol 3-phosphate in sporulation of Schizosaccharomyces pombe

Takako Koga, Masayuki Onishi, Yoko Nakamura, Aiko Hirata, Taro Nakamura, Chikashi Shimoda, Tomoko Iwaki, Kaoru Takegawa, Yasushisa Fukui

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Schizosaccharomyces pombe defective in phosphatidylinositol (PtdIns) 3-kinase shows various defects in forespore membrane formation, including onset, growth orientation, and closure. Downstream factors of PtdIns 3-kinase in this system were explored. Among various phox homology (PX) domain-containing proteins, Vps5p and Vps17p, homologues of sorting nexins, were found to be required for efficient sporulation. Cells defective in these proteins showed a disordered growth orientation of the forespore membrane, as is the case with Δpik3 cells. Vps5p and Vps17p with mutations in the PX domains failed to suppress the defects of their relevant disruptants. Vps5p and Vps17p migrated toward the the forespore membrane in a pik3+-dependent manner, suggesting that these proteins may interact with PtdIns(3)P. Electron-microscopic analysis revealed that the forespore membrane fails to engulf the nucleus in some of these cells, accumulating vesicle-like bodies similar to those seen in Δspo3 cells. These results suggest that Vps5p and Vps17p are the targets of PtdIns(3)P in vesicle transport required for onset of the forespore membrane formation.

Original languageEnglish
Pages (from-to)561-574
Number of pages14
JournalGenes to Cells
Volume9
Issue number6
DOIs
Publication statusPublished - Jun 1 2004
Externally publishedYes

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Sorting Nexins
Schizosaccharomyces
Membranes
Phosphatidylinositol 3-Kinase
Phosphatidylinositols
Transport Vesicles
Growth
Proteins
phosphatidylinositol 3-phosphate
Electrons
Mutation

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Sorting nexin homologues are targets of phosphatidylinositol 3-phosphate in sporulation of Schizosaccharomyces pombe. / Koga, Takako; Onishi, Masayuki; Nakamura, Yoko; Hirata, Aiko; Nakamura, Taro; Shimoda, Chikashi; Iwaki, Tomoko; Takegawa, Kaoru; Fukui, Yasushisa.

In: Genes to Cells, Vol. 9, No. 6, 01.06.2004, p. 561-574.

Research output: Contribution to journalArticle

Koga, T, Onishi, M, Nakamura, Y, Hirata, A, Nakamura, T, Shimoda, C, Iwaki, T, Takegawa, K & Fukui, Y 2004, 'Sorting nexin homologues are targets of phosphatidylinositol 3-phosphate in sporulation of Schizosaccharomyces pombe', Genes to Cells, vol. 9, no. 6, pp. 561-574. https://doi.org/10.1111/j.1356-9597.2004.00744.x
Koga, Takako ; Onishi, Masayuki ; Nakamura, Yoko ; Hirata, Aiko ; Nakamura, Taro ; Shimoda, Chikashi ; Iwaki, Tomoko ; Takegawa, Kaoru ; Fukui, Yasushisa. / Sorting nexin homologues are targets of phosphatidylinositol 3-phosphate in sporulation of Schizosaccharomyces pombe. In: Genes to Cells. 2004 ; Vol. 9, No. 6. pp. 561-574.
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