Sour taste responses in mice lacking pkd channels

Nao Horio, Ryusuke Yoshida, Keiko Nakano, Yuchio Yanagawa, Yoshiro Ishimaru, Hiroaki Matsunami, Yuzo Ninomiya

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Background: The polycystic kidney disease-like ion channel PKD2L1 and its associated partner PKD1L3 are potential candidates for sour taste receptors. PKD2L1 is expressed in type III taste cells that respond to sour stimuli and genetic elimination of cells expressing PKD2L1 substantially reduces chorda tympani nerve responses to sour taste stimuli. However, the contribution of PKD2L1 and PKD1L3 to sour taste responses remains unclear. Methodology/Principal Findings: We made mice lacking PKD2L1 and/or PKD1L3 gene and investigated whole nerve responses to taste stimuli in the chorda tympani or the glossopharyngeal nerve and taste responses in type III taste cells. In mice lacking PKD2L1 gene, chorda tympani nerve responses to sour, but not sweet, salty, bitter, and umami tastants were reduced by 25-45% compared with those in wild type mice. In contrast, chorda tympani nerve responses in PKD1L3 knock-out mice and glossopharyngeal nerve responses in single- and double-knock-out mice were similar to those in wild type mice. Sour taste responses of type III fungiform taste cells (GAD67-expressing taste cells) were also reduced by 25-45% by elimination of PKD2L1. Conclusions/Significance: These findings suggest that PKD2L1 partly contributes to sour taste responses in mice and that receptors other than PKDs would be involved in sour detection.

Original languageEnglish
Article numbere20007
JournalPloS one
Volume6
Issue number5
DOIs
Publication statusPublished - May 25 2011

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Genes
Tympanis
mice
Ion Channels
nerve tissue
Chorda Tympani Nerve
Cells
Glossopharyngeal Nerve
cells
Knockout Mice
umami
Polycystic Kidney Diseases
ion channels
genes
receptors
Chorda

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Horio, N., Yoshida, R., Nakano, K., Yanagawa, Y., Ishimaru, Y., Matsunami, H., & Ninomiya, Y. (2011). Sour taste responses in mice lacking pkd channels. PloS one, 6(5), [e20007]. https://doi.org/10.1371/journal.pone.0020007

Sour taste responses in mice lacking pkd channels. / Horio, Nao; Yoshida, Ryusuke; Nakano, Keiko; Yanagawa, Yuchio; Ishimaru, Yoshiro; Matsunami, Hiroaki; Ninomiya, Yuzo.

In: PloS one, Vol. 6, No. 5, e20007, 25.05.2011.

Research output: Contribution to journalArticle

Horio, N, Yoshida, R, Nakano, K, Yanagawa, Y, Ishimaru, Y, Matsunami, H & Ninomiya, Y 2011, 'Sour taste responses in mice lacking pkd channels', PloS one, vol. 6, no. 5, e20007. https://doi.org/10.1371/journal.pone.0020007
Horio N, Yoshida R, Nakano K, Yanagawa Y, Ishimaru Y, Matsunami H et al. Sour taste responses in mice lacking pkd channels. PloS one. 2011 May 25;6(5). e20007. https://doi.org/10.1371/journal.pone.0020007
Horio, Nao ; Yoshida, Ryusuke ; Nakano, Keiko ; Yanagawa, Yuchio ; Ishimaru, Yoshiro ; Matsunami, Hiroaki ; Ninomiya, Yuzo. / Sour taste responses in mice lacking pkd channels. In: PloS one. 2011 ; Vol. 6, No. 5.
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