Southern blotting analyses of strains of Campylobacter fetus using the conserved region of sapA

Masaki Fujita; Tatsuya Morooka; Shuji Fujimoto; Tetsuhiro Moriya; Kazunobu Amako

doi:10.1007/BF02529743

Southern blotting analyses of strains of Campylobacter fetus using the conserved region of sapA

Masaki Fujita, Tatsuya Morooka, Shuji Fujimoto, Tetsuhiro Moriya, Kazunobu Amako

Division of Medical Technology

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Chromosomal DNA of 27 strains of Campylobacter fetus was analyzed by Southern blotting with a probe of the conserved region of sapA. The probe hybridized with 23 strains that produced type A lipopolysaccharide. These strains had more than six sapA homologs. In Southern blots of SalI-digested chromosomal DNA separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. These data indicate that multiple sapA homologs are localized to a limited region on the chromosomal DNA of C. fetus and thus suggest the possibility of developing a typing system using this method.

Original language	English
Pages (from-to)	444-447
Number of pages	4
Journal	Archives of Microbiology
Volume	164
Issue number	6
DOIs	https://doi.org/10.1007/BF02529743
Publication status	Published - Dec 1 1995

All Science Journal Classification (ASJC) codes

Microbiology
Biochemistry
Molecular Biology
Genetics

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title = "Southern blotting analyses of strains of Campylobacter fetus using the conserved region of sapA",

abstract = "Chromosomal DNA of 27 strains of Campylobacter fetus was analyzed by Southern blotting with a probe of the conserved region of sapA. The probe hybridized with 23 strains that produced type A lipopolysaccharide. These strains had more than six sapA homologs. In Southern blots of SalI-digested chromosomal DNA separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. These data indicate that multiple sapA homologs are localized to a limited region on the chromosomal DNA of C. fetus and thus suggest the possibility of developing a typing system using this method.",

author = "Masaki Fujita and Tatsuya Morooka and Shuji Fujimoto and Tetsuhiro Moriya and Kazunobu Amako",

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T1 - Southern blotting analyses of strains of Campylobacter fetus using the conserved region of sapA

AU - Fujita, Masaki

AU - Morooka, Tatsuya

AU - Fujimoto, Shuji

AU - Moriya, Tetsuhiro

AU - Amako, Kazunobu

PY - 1995/12/1

Y1 - 1995/12/1

N2 - Chromosomal DNA of 27 strains of Campylobacter fetus was analyzed by Southern blotting with a probe of the conserved region of sapA. The probe hybridized with 23 strains that produced type A lipopolysaccharide. These strains had more than six sapA homologs. In Southern blots of SalI-digested chromosomal DNA separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. These data indicate that multiple sapA homologs are localized to a limited region on the chromosomal DNA of C. fetus and thus suggest the possibility of developing a typing system using this method.

AB - Chromosomal DNA of 27 strains of Campylobacter fetus was analyzed by Southern blotting with a probe of the conserved region of sapA. The probe hybridized with 23 strains that produced type A lipopolysaccharide. These strains had more than six sapA homologs. In Southern blots of SalI-digested chromosomal DNA separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. These data indicate that multiple sapA homologs are localized to a limited region on the chromosomal DNA of C. fetus and thus suggest the possibility of developing a typing system using this method.

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JF - Archives of Microbiology

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ER -

Southern blotting analyses of strains of Campylobacter fetus using the conserved region of sapA

Abstract

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