Abstract
Chromosomal DNA of 27 strains of Campylobacter fetus was analyzed by Southern blotting with a probe of the conserved region of sapA. The probe hybridized with 23 strains that produced type A lipopolysaccharide. These strains had more than six sapA homologs. In Southern blots of SalI-digested chromosomal DNA separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. These data indicate that multiple sapA homologs are localized to a limited region on the chromosomal DNA of C. fetus and thus suggest the possibility of developing a typing system using this method.
| Original language | English |
|---|---|
| Pages (from-to) | 444-447 |
| Number of pages | 4 |
| Journal | Archives of Microbiology |
| Volume | 164 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 1 1995 |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Biochemistry
- Molecular Biology
- Genetics
Cite this
Southern blotting analyses of strains of Campylobacter fetus using the conserved region of sapA. / Fujita, Masaki; Morooka, Tatsuya; Fujimoto, Shuji; Moriya, Tetsuhiro; Amako, Kazunobu.
In: Archives of Microbiology, Vol. 164, No. 6, 01.12.1995, p. 444-447.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Southern blotting analyses of strains of Campylobacter fetus using the conserved region of sapA
AU - Fujita, Masaki
AU - Morooka, Tatsuya
AU - Fujimoto, Shuji
AU - Moriya, Tetsuhiro
AU - Amako, Kazunobu
PY - 1995/12/1
Y1 - 1995/12/1
N2 - Chromosomal DNA of 27 strains of Campylobacter fetus was analyzed by Southern blotting with a probe of the conserved region of sapA. The probe hybridized with 23 strains that produced type A lipopolysaccharide. These strains had more than six sapA homologs. In Southern blots of SalI-digested chromosomal DNA separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. These data indicate that multiple sapA homologs are localized to a limited region on the chromosomal DNA of C. fetus and thus suggest the possibility of developing a typing system using this method.
AB - Chromosomal DNA of 27 strains of Campylobacter fetus was analyzed by Southern blotting with a probe of the conserved region of sapA. The probe hybridized with 23 strains that produced type A lipopolysaccharide. These strains had more than six sapA homologs. In Southern blots of SalI-digested chromosomal DNA separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. These data indicate that multiple sapA homologs are localized to a limited region on the chromosomal DNA of C. fetus and thus suggest the possibility of developing a typing system using this method.
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UR - http://www.scopus.com/inward/citedby.url?scp=0029615383&partnerID=8YFLogxK
U2 - 10.1007/BF02529743
DO - 10.1007/BF02529743
M3 - Article
VL - 164
SP - 444
EP - 447
JO - Archives of Microbiology
JF - Archives of Microbiology
SN - 0302-8933
IS - 6
ER -