Southern blotting analyses of strains of Campylobacter fetus using the conserved region of sapA

Masaki Fujita, Tatsuya Morooka, Shuji Fujimoto, Tetsuhiro Moriya, Kazunobu Amako

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Chromosomal DNA of 27 strains of Campylobacter fetus was analyzed by Southern blotting with a probe of the conserved region of sapA. The probe hybridized with 23 strains that produced type A lipopolysaccharide. These strains had more than six sapA homologs. In Southern blots of SalI-digested chromosomal DNA separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. These data indicate that multiple sapA homologs are localized to a limited region on the chromosomal DNA of C. fetus and thus suggest the possibility of developing a typing system using this method.

Original languageEnglish
Pages (from-to)444-447
Number of pages4
JournalArchives of Microbiology
Volume164
Issue number6
DOIs
Publication statusPublished - Dec 1 1995

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

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