Southern blotting analyses of strains of Campylobacter fetus using the conserved region of sapA

Masaki Fujita, Tatsuya Morooka, Shuji Fujimoto, Tetsuhiro Moriya, Kazunobu Amako

Research output: Contribution to journalArticle

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Abstract

Chromosomal DNA of 27 strains of Campylobacter fetus was analyzed by Southern blotting with a probe of the conserved region of sapA. The probe hybridized with 23 strains that produced type A lipopolysaccharide. These strains had more than six sapA homologs. In Southern blots of SalI-digested chromosomal DNA separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. These data indicate that multiple sapA homologs are localized to a limited region on the chromosomal DNA of C. fetus and thus suggest the possibility of developing a typing system using this method.

Original languageEnglish
Pages (from-to)444-447
Number of pages4
JournalArchives of Microbiology
Volume164
Issue number6
DOIs
Publication statusPublished - Dec 1 1995

Fingerprint

Campylobacter fetus
Southern Blotting
DNA
Pulsed Field Gel Electrophoresis
Electrophoresis
Lipopolysaccharides
Gels

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Southern blotting analyses of strains of Campylobacter fetus using the conserved region of sapA. / Fujita, Masaki; Morooka, Tatsuya; Fujimoto, Shuji; Moriya, Tetsuhiro; Amako, Kazunobu.

In: Archives of Microbiology, Vol. 164, No. 6, 01.12.1995, p. 444-447.

Research output: Contribution to journalArticle

Fujita, Masaki ; Morooka, Tatsuya ; Fujimoto, Shuji ; Moriya, Tetsuhiro ; Amako, Kazunobu. / Southern blotting analyses of strains of Campylobacter fetus using the conserved region of sapA. In: Archives of Microbiology. 1995 ; Vol. 164, No. 6. pp. 444-447.
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