Abstract
Chromosomal DNA of 27 strains of Campylobacter fetus was analyzed by Southern blotting with a probe of the conserved region of sapA. The probe hybridized with 23 strains that produced type A lipopolysaccharide. These strains had more than six sapA homologs. In Southern blots of SalI-digested chromosomal DNA separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. These data indicate that multiple sapA homologs are localized to a limited region on the chromosomal DNA of C. fetus and thus suggest the possibility of developing a typing system using this method.
| Original language | English |
|---|---|
| Pages (from-to) | 444-447 |
| Number of pages | 4 |
| Journal | Archives of Microbiology |
| Volume | 164 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 1 1995 |
All Science Journal Classification (ASJC) codes
- Microbiology
- Biochemistry
- Molecular Biology
- Genetics
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Fujita, M., Morooka, T., Fujimoto, S., Moriya, T., & Amako, K. (1995). Southern blotting analyses of strains of Campylobacter fetus using the conserved region of sapA. Archives of Microbiology, 164(6), 444-447. https://doi.org/10.1007/BF02529743