Soybean-Derived Glycine-Arginine Dipeptide Administration Promotes Neurotrophic Factor Expression in the Mouse Brain

Ayano Shimizu, Takakazu Mitani, Sachi Tanaka, Hiroshi Fujii, Motohiro Maebuchi, Yusuke Amiya, Mitsuru Tanaka, Toshiro Matsui, Soichiro Nakamura, Shigeru Katayama

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, plays an important role in cognitive abilities, including memory and learning. We demonstrated that soybean protein hydrolysate (SPH) diet suppresses age-related cognitive decline via the upregulation of BDNF in a mouse model of senescence. Our purpose was to identify novel bioactive peptides in SPH, which enhance BDNF expression. We treated mouse primary astrocytes with SPH as well as with its positively charged chromatographic fraction. Significant increases in the expression of BDNF were observed in the treatment with positively charged fraction of SPH. Among the synthesized peptides, the dipeptide glycine-arginine (GR) increased BDNF expression in vitro, and LC-TOF-MS analysis showed the presence of GR in the SPH. Furthermore, its administration in vivo increased the expression of BDNF in the cerebral cortex and the number of neurons in hippocampus and cerebral cortex. These data indicate that GR might promote neurogenesis by upregulating BDNF levels.

Original languageEnglish
Pages (from-to)7935-7941
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume66
Issue number30
DOIs
Publication statusPublished - Aug 1 2018

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Glycine (Fabaceae)
neurotrophins
dipeptides
Dipeptides
Brain-Derived Neurotrophic Factor
Nerve Growth Factors
Soybeans
Protein Hydrolysates
Glycine
arginine
Arginine
Soybean Proteins
Brain
soybeans
protein hydrolysates
brain
soy protein
mice
cerebral cortex
Cerebral Cortex

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Soybean-Derived Glycine-Arginine Dipeptide Administration Promotes Neurotrophic Factor Expression in the Mouse Brain. / Shimizu, Ayano; Mitani, Takakazu; Tanaka, Sachi; Fujii, Hiroshi; Maebuchi, Motohiro; Amiya, Yusuke; Tanaka, Mitsuru; Matsui, Toshiro; Nakamura, Soichiro; Katayama, Shigeru.

In: Journal of Agricultural and Food Chemistry, Vol. 66, No. 30, 01.08.2018, p. 7935-7941.

Research output: Contribution to journalArticle

Shimizu, Ayano ; Mitani, Takakazu ; Tanaka, Sachi ; Fujii, Hiroshi ; Maebuchi, Motohiro ; Amiya, Yusuke ; Tanaka, Mitsuru ; Matsui, Toshiro ; Nakamura, Soichiro ; Katayama, Shigeru. / Soybean-Derived Glycine-Arginine Dipeptide Administration Promotes Neurotrophic Factor Expression in the Mouse Brain. In: Journal of Agricultural and Food Chemistry. 2018 ; Vol. 66, No. 30. pp. 7935-7941.
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AU - Fujii, Hiroshi

AU - Maebuchi, Motohiro

AU - Amiya, Yusuke

AU - Tanaka, Mitsuru

AU - Matsui, Toshiro

AU - Nakamura, Soichiro

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