Soybean preference for Bradyrhizobium japonicum for nodulation: Occupation of serogroup USDA110 in Nodules of soybean plants harboring various Rj-genes grown in a field

Takeo Yamakawa, Abul Kashem Mohammed Anuwar Hussain, Junji Ishizuka, Junji Ishizuka

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

For examining the probability of increase in the occupation ratio of inoculated rhizobium in nodules, various Rj-soybean cultivars including the Rj2Rj3Rj4-lines of soybean were grown in a field of the Kyushu University Farm. Bradyrhizobium japonicum USDA110 that carries uptake hydrogenase (Hup+) was used as an inoculum. The relative efficiency of nitrogen fixation generally increased by the inoculation. However, there were no significant differences in the effects among the genotypes of the host plants. The occupation ratio of serogroup USDA110 in the nodules on the taproot of the inoculated plants was in the range of 77–100%, suggesting that the B. japonicum strain USDA110 infected taproots immediately after inoculation. The occupation ratios in the nodules on the lateral roots were 53–67, 40–86, 63–83, and 62–77% in inoculated plants of the non-Rj-, Rj2Rj3-, Rj4-, and Rj2Rj3Rj4-genotypes, respectively, and they decreased in all the genotypes with the progression of growth. At the time of the first sampling, the occupation ratios on the lateral roots of these Rj2Rj3Rj4-genotypes showed values intermediate between those of IAC-2 (Rj2Rj3) and Hill (Rj4), which were the parent cultivars of the Rj2Rj3Rj4-lines, B340, B349, and C242. The reduction in the occupation ratio of the serogroup USDA110 for about 1 month after the first sampling was the lowest (0.13–0.16) in the Rj2Rj3Rj4-genotypes, excluding B349, followed by the non-Rj- and Rj2Rj3-genotypes and highest (0.52–0.69) in the Rj4-genotypes, excluding Hill. Therefore, it was considered that the population of compatible rhizobia with host soybean plants increased in the rhizosphere with the progression of the development and growth. The results showed that with the expansion of the root area of host plants, the occupation ratio of type A rhizobia including the serogroup USDA110 was high. Therefore, the Rj2Rj3Rj4-genotypes were superior to other Rj-genotypes in terms of the inoculation effects of nodulation type A rhizobium, B. japonicum USDA110. However, the preference of the Rj2Rj3Rj4-genotype for serogroup USDA110 is not sufficient to rule out the competition with the other serogroups in this study. Therefore, the study should be centered on the isolation of more efficient (Hup+) and highly compatible rhizobial strains with the Rj2Rj3Rj4- genotypes.

Original languageEnglish
Pages (from-to)835-841
Number of pages7
JournalSoil Science and Plant Nutrition
Volume49
Issue number6
DOIs
Publication statusPublished - Dec 1 2003

Fingerprint

Bradyrhizobium japonicum
nodulation
occupation
soybean
serotypes
genotype
soybeans
gene
genes
rhizobacterium
Rhizobium
inoculation
host plant
cultivar
host plants
sampling
cultivars
nitrogen fixation
growth and development
rhizosphere

All Science Journal Classification (ASJC) codes

  • Soil Science
  • Plant Science

Cite this

Soybean preference for Bradyrhizobium japonicum for nodulation : Occupation of serogroup USDA110 in Nodules of soybean plants harboring various Rj-genes grown in a field. / Yamakawa, Takeo; Hussain, Abul Kashem Mohammed Anuwar; Ishizuka, Junji; Ishizuka, Junji.

In: Soil Science and Plant Nutrition, Vol. 49, No. 6, 01.12.2003, p. 835-841.

Research output: Contribution to journalArticle

@article{dd0bdc5cc8d94521ac742d248486737a,
title = "Soybean preference for Bradyrhizobium japonicum for nodulation: Occupation of serogroup USDA110 in Nodules of soybean plants harboring various Rj-genes grown in a field",
abstract = "For examining the probability of increase in the occupation ratio of inoculated rhizobium in nodules, various Rj-soybean cultivars including the Rj2Rj3Rj4-lines of soybean were grown in a field of the Kyushu University Farm. Bradyrhizobium japonicum USDA110 that carries uptake hydrogenase (Hup+) was used as an inoculum. The relative efficiency of nitrogen fixation generally increased by the inoculation. However, there were no significant differences in the effects among the genotypes of the host plants. The occupation ratio of serogroup USDA110 in the nodules on the taproot of the inoculated plants was in the range of 77–100{\%}, suggesting that the B. japonicum strain USDA110 infected taproots immediately after inoculation. The occupation ratios in the nodules on the lateral roots were 53–67, 40–86, 63–83, and 62–77{\%} in inoculated plants of the non-Rj-, Rj2Rj3-, Rj4-, and Rj2Rj3Rj4-genotypes, respectively, and they decreased in all the genotypes with the progression of growth. At the time of the first sampling, the occupation ratios on the lateral roots of these Rj2Rj3Rj4-genotypes showed values intermediate between those of IAC-2 (Rj2Rj3) and Hill (Rj4), which were the parent cultivars of the Rj2Rj3Rj4-lines, B340, B349, and C242. The reduction in the occupation ratio of the serogroup USDA110 for about 1 month after the first sampling was the lowest (0.13–0.16) in the Rj2Rj3Rj4-genotypes, excluding B349, followed by the non-Rj- and Rj2Rj3-genotypes and highest (0.52–0.69) in the Rj4-genotypes, excluding Hill. Therefore, it was considered that the population of compatible rhizobia with host soybean plants increased in the rhizosphere with the progression of the development and growth. The results showed that with the expansion of the root area of host plants, the occupation ratio of type A rhizobia including the serogroup USDA110 was high. Therefore, the Rj2Rj3Rj4-genotypes were superior to other Rj-genotypes in terms of the inoculation effects of nodulation type A rhizobium, B. japonicum USDA110. However, the preference of the Rj2Rj3Rj4-genotype for serogroup USDA110 is not sufficient to rule out the competition with the other serogroups in this study. Therefore, the study should be centered on the isolation of more efficient (Hup+) and highly compatible rhizobial strains with the Rj2Rj3Rj4- genotypes.",
author = "Takeo Yamakawa and Hussain, {Abul Kashem Mohammed Anuwar} and Junji Ishizuka and Junji Ishizuka",
year = "2003",
month = "12",
day = "1",
doi = "10.1080/00380768.2003.10410345",
language = "English",
volume = "49",
pages = "835--841",
journal = "Soil Science and Plant Nutrition",
issn = "0038-0768",
publisher = "Taylor and Francis Ltd.",
number = "6",

}

TY - JOUR

T1 - Soybean preference for Bradyrhizobium japonicum for nodulation

T2 - Occupation of serogroup USDA110 in Nodules of soybean plants harboring various Rj-genes grown in a field

AU - Yamakawa, Takeo

AU - Hussain, Abul Kashem Mohammed Anuwar

AU - Ishizuka, Junji

AU - Ishizuka, Junji

PY - 2003/12/1

Y1 - 2003/12/1

N2 - For examining the probability of increase in the occupation ratio of inoculated rhizobium in nodules, various Rj-soybean cultivars including the Rj2Rj3Rj4-lines of soybean were grown in a field of the Kyushu University Farm. Bradyrhizobium japonicum USDA110 that carries uptake hydrogenase (Hup+) was used as an inoculum. The relative efficiency of nitrogen fixation generally increased by the inoculation. However, there were no significant differences in the effects among the genotypes of the host plants. The occupation ratio of serogroup USDA110 in the nodules on the taproot of the inoculated plants was in the range of 77–100%, suggesting that the B. japonicum strain USDA110 infected taproots immediately after inoculation. The occupation ratios in the nodules on the lateral roots were 53–67, 40–86, 63–83, and 62–77% in inoculated plants of the non-Rj-, Rj2Rj3-, Rj4-, and Rj2Rj3Rj4-genotypes, respectively, and they decreased in all the genotypes with the progression of growth. At the time of the first sampling, the occupation ratios on the lateral roots of these Rj2Rj3Rj4-genotypes showed values intermediate between those of IAC-2 (Rj2Rj3) and Hill (Rj4), which were the parent cultivars of the Rj2Rj3Rj4-lines, B340, B349, and C242. The reduction in the occupation ratio of the serogroup USDA110 for about 1 month after the first sampling was the lowest (0.13–0.16) in the Rj2Rj3Rj4-genotypes, excluding B349, followed by the non-Rj- and Rj2Rj3-genotypes and highest (0.52–0.69) in the Rj4-genotypes, excluding Hill. Therefore, it was considered that the population of compatible rhizobia with host soybean plants increased in the rhizosphere with the progression of the development and growth. The results showed that with the expansion of the root area of host plants, the occupation ratio of type A rhizobia including the serogroup USDA110 was high. Therefore, the Rj2Rj3Rj4-genotypes were superior to other Rj-genotypes in terms of the inoculation effects of nodulation type A rhizobium, B. japonicum USDA110. However, the preference of the Rj2Rj3Rj4-genotype for serogroup USDA110 is not sufficient to rule out the competition with the other serogroups in this study. Therefore, the study should be centered on the isolation of more efficient (Hup+) and highly compatible rhizobial strains with the Rj2Rj3Rj4- genotypes.

AB - For examining the probability of increase in the occupation ratio of inoculated rhizobium in nodules, various Rj-soybean cultivars including the Rj2Rj3Rj4-lines of soybean were grown in a field of the Kyushu University Farm. Bradyrhizobium japonicum USDA110 that carries uptake hydrogenase (Hup+) was used as an inoculum. The relative efficiency of nitrogen fixation generally increased by the inoculation. However, there were no significant differences in the effects among the genotypes of the host plants. The occupation ratio of serogroup USDA110 in the nodules on the taproot of the inoculated plants was in the range of 77–100%, suggesting that the B. japonicum strain USDA110 infected taproots immediately after inoculation. The occupation ratios in the nodules on the lateral roots were 53–67, 40–86, 63–83, and 62–77% in inoculated plants of the non-Rj-, Rj2Rj3-, Rj4-, and Rj2Rj3Rj4-genotypes, respectively, and they decreased in all the genotypes with the progression of growth. At the time of the first sampling, the occupation ratios on the lateral roots of these Rj2Rj3Rj4-genotypes showed values intermediate between those of IAC-2 (Rj2Rj3) and Hill (Rj4), which were the parent cultivars of the Rj2Rj3Rj4-lines, B340, B349, and C242. The reduction in the occupation ratio of the serogroup USDA110 for about 1 month after the first sampling was the lowest (0.13–0.16) in the Rj2Rj3Rj4-genotypes, excluding B349, followed by the non-Rj- and Rj2Rj3-genotypes and highest (0.52–0.69) in the Rj4-genotypes, excluding Hill. Therefore, it was considered that the population of compatible rhizobia with host soybean plants increased in the rhizosphere with the progression of the development and growth. The results showed that with the expansion of the root area of host plants, the occupation ratio of type A rhizobia including the serogroup USDA110 was high. Therefore, the Rj2Rj3Rj4-genotypes were superior to other Rj-genotypes in terms of the inoculation effects of nodulation type A rhizobium, B. japonicum USDA110. However, the preference of the Rj2Rj3Rj4-genotype for serogroup USDA110 is not sufficient to rule out the competition with the other serogroups in this study. Therefore, the study should be centered on the isolation of more efficient (Hup+) and highly compatible rhizobial strains with the Rj2Rj3Rj4- genotypes.

UR - http://www.scopus.com/inward/record.url?scp=0345770115&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345770115&partnerID=8YFLogxK

U2 - 10.1080/00380768.2003.10410345

DO - 10.1080/00380768.2003.10410345

M3 - Article

AN - SCOPUS:0345770115

VL - 49

SP - 835

EP - 841

JO - Soil Science and Plant Nutrition

JF - Soil Science and Plant Nutrition

SN - 0038-0768

IS - 6

ER -