TY - JOUR
T1 - Spatial patterns of sucrose-inducible and polygalacturonic acid-inducible expression of genes that encode sporamin and rβ-amylase in sweet potato
AU - Takeda, Shin
AU - Kowyama, Yasuo
AU - Takeuchi, Yuka
AU - Matsuoka, Ken
AU - Nishimura, Mikio
N1 - Funding Information:
The authors thank Mr. Tomiji Izuhara and Dr. Shigekata Yoshida of Nagoya University Experimental Farm for growing the sweet potato plants and Dr. Teiji Kokubu of the School of Agriculture, Kagoshima University, for valuable suggestions. This work was supported in part by Orants-in-Aid for Scientific Research on Priority Areas ("Molecular Mechanism of Plasticity and Signal Response in Plant Gene Expression", No. 0425102 and "The Molecular Basis of Flexible Organ Plans in Plants", No. 06278102) from the Ministry of Education, Science and Culture of Japan.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1995/3
Y1 - 1995/3
N2 - Two major proteins of tuberous roots of sweet potato, sporamin and rβ-amylase, were detected in storage parenchyma cells, which contain a large amount of starch. In both the leaves and petioles of sweet potato, the sucrose-induced accumulation of mRNAs for sporamin and rβ-amylase, and of starch occurred in a wide variety of cells, first in cells within and around the vascular tissue and then in various cells distal to them, with the exception of epidermal cells. In the mesophyll cells of leaves treated with sucrose, the accumulation of large numbers of well-developed starch granules occurred in the preexisting chloroplasts. These results, together with the previous observation that the sucrose-induced accumulation of sporamin, of rβ-amylase and of starch occurs with similar dependency on the concentration of sucrose, suggest that an excess supply of sugars to various types of cell triggers a cellular transition that induces the simultaneous accumulation of these reserve materials that are normally present in tuberous roots. Accumulation of mRNAs for sporamin and rβ-amylase, but not the accumulation of starch, in leaves and petioles can be also induced when leaf-petiole cuttings are supplied with low concentrations of polygalacturonic acid (PGA) at their cut edges. The spatial patterns of accumulation of mRNAs for sporamin and rβ-amylase in leaves and petioles after treatment with PGA were found to be similar to those observed upon treatment with sucrose. These results suggest that most of the cells in leaves and petioles have the capacity to respond to both a carbohydrate metabolic signal and a PGA-derived signal that is transmitted by diffusion from the vascular system.
AB - Two major proteins of tuberous roots of sweet potato, sporamin and rβ-amylase, were detected in storage parenchyma cells, which contain a large amount of starch. In both the leaves and petioles of sweet potato, the sucrose-induced accumulation of mRNAs for sporamin and rβ-amylase, and of starch occurred in a wide variety of cells, first in cells within and around the vascular tissue and then in various cells distal to them, with the exception of epidermal cells. In the mesophyll cells of leaves treated with sucrose, the accumulation of large numbers of well-developed starch granules occurred in the preexisting chloroplasts. These results, together with the previous observation that the sucrose-induced accumulation of sporamin, of rβ-amylase and of starch occurs with similar dependency on the concentration of sucrose, suggest that an excess supply of sugars to various types of cell triggers a cellular transition that induces the simultaneous accumulation of these reserve materials that are normally present in tuberous roots. Accumulation of mRNAs for sporamin and rβ-amylase, but not the accumulation of starch, in leaves and petioles can be also induced when leaf-petiole cuttings are supplied with low concentrations of polygalacturonic acid (PGA) at their cut edges. The spatial patterns of accumulation of mRNAs for sporamin and rβ-amylase in leaves and petioles after treatment with PGA were found to be similar to those observed upon treatment with sucrose. These results suggest that most of the cells in leaves and petioles have the capacity to respond to both a carbohydrate metabolic signal and a PGA-derived signal that is transmitted by diffusion from the vascular system.
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U2 - 10.1093/oxfordjournals.pcp.a078764
DO - 10.1093/oxfordjournals.pcp.a078764
M3 - Article
AN - SCOPUS:0029169548
VL - 36
SP - 321
EP - 333
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 2
ER -