Spatiotemporal control of cell adhesion on a self-assembled monolayer having a photocleavable protecting group

Jun Nakanishi, Yukiko Kikuchi, Tohru Takarada, Hidekazu Nakayama, Kazuo Yamaguchi, Mizuo Maeda

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Control of cell adhesion is a key technology for cell-based drug screening and for analyses of cellular processes. We developed a method to spatiotemporally control cell adhesion using a photochemical reaction. We prepared a cell-culturing substrate by modifying the surface of a glass coverslip with a self-assembled monolayer of an alkylsiloxane having a photocleavable 2-nitrobenzyl group. Bovine serum albumin (BSA) was adsorbed onto the substrate to make the surface inert to cell adhesion. When exposed to UV light, the alkylsiloxane underwent a photocleavage reaction, leading to the release of BSA from the surface. Fibronectin, a protein promoting cell adhesion, was added to cover the irradiated regions and made them cell-adhesive. Seeding of cells on this substrate resulted in their selective adhesion to the illuminated regions. By controlling the sizes of the illuminated regions, we formed cell-adhesive spots smaller than single cells and located focal adhesions of the cells. Moreover, by subsequently illuminating the region alongside the cells patterned on the substrate in advance, we released their geometrical confinements and induced migration and proliferation. These manipulations were conducted under a conventional fluorescence microscope without any additional instruments. The present method of cell manipulation will be useful for cell biological studies as well as for the formation of cell arrays.

Original languageEnglish
Pages (from-to)100-104
Number of pages5
JournalAnalytica Chimica Acta
Volume578
Issue number1
DOIs
Publication statusPublished - Sep 18 2006
Externally publishedYes

Fingerprint

Cell adhesion
Self assembled monolayers
adhesion
Cell Adhesion
Substrates
Bovine Serum Albumin
substrate
Adhesives
Adhesion
Cells
serum
Photochemical reactions
Fibronectins
Ultraviolet radiation
Screening
Microscopes
Fluorescence
seeding
Glass
drug

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

Spatiotemporal control of cell adhesion on a self-assembled monolayer having a photocleavable protecting group. / Nakanishi, Jun; Kikuchi, Yukiko; Takarada, Tohru; Nakayama, Hidekazu; Yamaguchi, Kazuo; Maeda, Mizuo.

In: Analytica Chimica Acta, Vol. 578, No. 1, 18.09.2006, p. 100-104.

Research output: Contribution to journalArticle

Nakanishi, Jun ; Kikuchi, Yukiko ; Takarada, Tohru ; Nakayama, Hidekazu ; Yamaguchi, Kazuo ; Maeda, Mizuo. / Spatiotemporal control of cell adhesion on a self-assembled monolayer having a photocleavable protecting group. In: Analytica Chimica Acta. 2006 ; Vol. 578, No. 1. pp. 100-104.
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