TY - JOUR
T1 - Spatiotemporal distribution of PKCα, Cdc42, and Rac1 before directed cell migration
AU - Sasaki, Saori
AU - Takahashi, Ryu
AU - Luo, Yangfeng
AU - Chujo, Kengo
AU - Sera, Toshihiro
AU - Kudo, Susumu
N1 - Funding Information:
This study was partially supported by JSPS Kakenhi (Grant No. JP16H02529 ) and Takahashi Industrial and Economic Research Foundation .
Publisher Copyright:
© 2021
PY - 2021/12/20
Y1 - 2021/12/20
N2 - Cdc42 is a key factor in directed cell migration and accumulates at the leading edge of migrating cells. However, what kind of proteins control Cdc42 and when is unclear. After mechanical wounding, protein kinase C α (PKCα), a conventional PKC isozyme, begins to accumulate at the edges of cells adjacent to the wounded cells (WCs). In this study, we hypothesized that PKCα may be implicated in directed cell migration at an early stage before Cdc42 controls the migration. We focused on the spatiotemporal distribution of PKCα, Cdc42, and Rac1 before cell migration. After wounding, at the edges of cells adjacent to the WCs, PKCα accumulation, Cdc42 accumulation, Rac1 accumulation, and filopodia formation occurred in that order. The PKCα inhibitor suppressed Cdc42 accumulation at the cell edges. These results suggest that inhibition of PKCα activity inhibits cell migration. In addition, it is not Cdc42 but PKCα that may decide the direction of cell migration.
AB - Cdc42 is a key factor in directed cell migration and accumulates at the leading edge of migrating cells. However, what kind of proteins control Cdc42 and when is unclear. After mechanical wounding, protein kinase C α (PKCα), a conventional PKC isozyme, begins to accumulate at the edges of cells adjacent to the wounded cells (WCs). In this study, we hypothesized that PKCα may be implicated in directed cell migration at an early stage before Cdc42 controls the migration. We focused on the spatiotemporal distribution of PKCα, Cdc42, and Rac1 before cell migration. After wounding, at the edges of cells adjacent to the WCs, PKCα accumulation, Cdc42 accumulation, Rac1 accumulation, and filopodia formation occurred in that order. The PKCα inhibitor suppressed Cdc42 accumulation at the cell edges. These results suggest that inhibition of PKCα activity inhibits cell migration. In addition, it is not Cdc42 but PKCα that may decide the direction of cell migration.
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U2 - 10.1016/j.bbrc.2021.10.080
DO - 10.1016/j.bbrc.2021.10.080
M3 - Article
C2 - 34753065
AN - SCOPUS:85118540051
VL - 584
SP - 26
EP - 31
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
ER -