Species identification in seven small millet species using polymerase chain reaction - Restriction fragment length polymorphism of trnS-psbC gene region

M. Parani, K. Rajesh, M. Lakshmi, L. Parducci, A. E. Szmidt, A. Parida

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The chloroplast trnS-psbC gene regions from total genomic DNA of 119 accessions from seven small millet species were amplified by polymerase chain reaction (PCR) and digested with eight restriction enzymes individually as well as in combinations of two enzymes to generate restriction fragment length polymorphism (PCR-RFLP). PCR-RFLP with individual enzymes revealed polymorphism between only some species. However, all the species could be distinguished by using a combination of two enzymes, specifically HaeIII and MspI. PCR-RFLP of 11 to 20 accessions with the same enzyme combination showed no intraspecific variation, which established that the differential banding patterns were species specific. In contrast, the same enzyme combination was not useful for differentiating different species of the genera Cajanus, Rhyncosia, Abies, Rhizophora, Ceriops, and Bruguiera, and it also revealed intraspecies variation in three species of Abies. The present study indicated that digestion of trnS-psbC with two four-base recognizing enzymes reveals more variation than with either enzyme alone and that it may be a method of choice for species identification in some genera.

Original languageEnglish
Pages (from-to)495-499
Number of pages5
JournalGenome
Volume44
Issue number3
DOIs
Publication statusPublished - Aug 29 2001

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Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction
Rhizophoraceae
Enzymes
Genes
Abies
Cajanus
Millets
Chloroplasts
Digestion
DNA

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Genetics

Cite this

Species identification in seven small millet species using polymerase chain reaction - Restriction fragment length polymorphism of trnS-psbC gene region. / Parani, M.; Rajesh, K.; Lakshmi, M.; Parducci, L.; Szmidt, A. E.; Parida, A.

In: Genome, Vol. 44, No. 3, 29.08.2001, p. 495-499.

Research output: Contribution to journalArticle

Parani, M. ; Rajesh, K. ; Lakshmi, M. ; Parducci, L. ; Szmidt, A. E. ; Parida, A. / Species identification in seven small millet species using polymerase chain reaction - Restriction fragment length polymorphism of trnS-psbC gene region. In: Genome. 2001 ; Vol. 44, No. 3. pp. 495-499.
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