Species identification of mustelids by comparing partial sequences on mitochondrial DNA from fecal samples

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14 Citations (Scopus)

Abstract

Nucleotide sequences of cytochrome b/tRNA/D-loop region on mitochondrial DNA of mustelids feces were compared to identify species. PCR amplification of target sequence for 47 (24.9%) feces and species identification of five feces (2.6%) out of 189 feces, collected at several study sites in Hokkaido, were successful. Species of three feces were Martes zibellina and those of other two feces were Martes melampus and Mustela itatsi. The low success rate of identification appeared to be due to failure of PCR amplification by inhibitors in feces. It was suggested that the method used in this study was useful for not only identify mustelids species, but also analyzing their genetic relationships.

Original languageEnglish
Pages (from-to)321-323
Number of pages3
JournalJournal of Veterinary Medical Science
Volume64
Issue number4
DOIs
Publication statusPublished - Apr 1 2002
Externally publishedYes

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Mitochondrial DNA
Feces
mitochondrial DNA
feces
Mustelidae
Martes
sampling
Mustela
Polymerase Chain Reaction
Cytochromes b
Transfer RNA
species identification
Faeces
cytochrome b
genetic relationships
Japan
nucleotide sequences

All Science Journal Classification (ASJC) codes

  • Marketing
  • veterinary(all)

Cite this

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abstract = "Nucleotide sequences of cytochrome b/tRNA/D-loop region on mitochondrial DNA of mustelids feces were compared to identify species. PCR amplification of target sequence for 47 (24.9{\%}) feces and species identification of five feces (2.6{\%}) out of 189 feces, collected at several study sites in Hokkaido, were successful. Species of three feces were Martes zibellina and those of other two feces were Martes melampus and Mustela itatsi. The low success rate of identification appeared to be due to failure of PCR amplification by inhibitors in feces. It was suggested that the method used in this study was useful for not only identify mustelids species, but also analyzing their genetic relationships.",
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AB - Nucleotide sequences of cytochrome b/tRNA/D-loop region on mitochondrial DNA of mustelids feces were compared to identify species. PCR amplification of target sequence for 47 (24.9%) feces and species identification of five feces (2.6%) out of 189 feces, collected at several study sites in Hokkaido, were successful. Species of three feces were Martes zibellina and those of other two feces were Martes melampus and Mustela itatsi. The low success rate of identification appeared to be due to failure of PCR amplification by inhibitors in feces. It was suggested that the method used in this study was useful for not only identify mustelids species, but also analyzing their genetic relationships.

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