Abstract
We investigated the effects of lactic acid bacterial (LAB) cells in rats using fluorescence in situ hybridization (FISH) targeting 16S rRNA to identify the cecal microbial community. We designed a novel species-specific 16S rDNA probe to detect Lactobacillus rhamnosus (Lrham454). Subtractive technique using the LAC722 probe (Sakai et al. 2004 Journal of Bioscience and Bioengineering 98, 48) under different hybridization stringency (LAC722(L-H)) was applied to identify Lactococcus lactis. We also applied Lplan447 and LAC722(L) to detect Lactobacillus plantarum and a wide range of LAB (total LAB), respectively. We optimized the hybridization and washing conditions and then quantified L. rhamnosus, L. plantarum, and L. lactis cells in rat cecal contents. We monitored increases in individual bacterial populations and in total LAB caused by the administration of the corresponding LAB cells. Growth, food efficiency and internal disorders did not significantly differ among the rats administered with LABs. Rats administered with polydextrose (POL) developed diarrhea, which decreased the total numbers of cecal bacteria, whereas the simultaneous administration of POL and L. rhamnosus KY-3 eased this symptom, and recovered the numbers of total LAB and of L. rhamnosus.
| Original language | English |
|---|---|
| Pages (from-to) | 493-499 |
| Number of pages | 7 |
| Journal | World Journal of Microbiology and Biotechnology |
| Volume | 22 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 1 2006 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Physiology
- Applied Microbiology and Biotechnology
Cite this
Species-specific FISH analysis of cecal microflora in rats administered with lactic acid bacteria. / Sakai, Kenji; Oue, Kazutoshi; Umeki, Miki; Mori, Masatsugu; Kuribayashi, Mari; Mochizuki, Satoshi.
In: World Journal of Microbiology and Biotechnology, Vol. 22, No. 5, 01.05.2006, p. 493-499.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Species-specific FISH analysis of cecal microflora in rats administered with lactic acid bacteria
AU - Sakai, Kenji
AU - Oue, Kazutoshi
AU - Umeki, Miki
AU - Mori, Masatsugu
AU - Kuribayashi, Mari
AU - Mochizuki, Satoshi
PY - 2006/5/1
Y1 - 2006/5/1
N2 - We investigated the effects of lactic acid bacterial (LAB) cells in rats using fluorescence in situ hybridization (FISH) targeting 16S rRNA to identify the cecal microbial community. We designed a novel species-specific 16S rDNA probe to detect Lactobacillus rhamnosus (Lrham454). Subtractive technique using the LAC722 probe (Sakai et al. 2004 Journal of Bioscience and Bioengineering 98, 48) under different hybridization stringency (LAC722(L-H)) was applied to identify Lactococcus lactis. We also applied Lplan447 and LAC722(L) to detect Lactobacillus plantarum and a wide range of LAB (total LAB), respectively. We optimized the hybridization and washing conditions and then quantified L. rhamnosus, L. plantarum, and L. lactis cells in rat cecal contents. We monitored increases in individual bacterial populations and in total LAB caused by the administration of the corresponding LAB cells. Growth, food efficiency and internal disorders did not significantly differ among the rats administered with LABs. Rats administered with polydextrose (POL) developed diarrhea, which decreased the total numbers of cecal bacteria, whereas the simultaneous administration of POL and L. rhamnosus KY-3 eased this symptom, and recovered the numbers of total LAB and of L. rhamnosus.
AB - We investigated the effects of lactic acid bacterial (LAB) cells in rats using fluorescence in situ hybridization (FISH) targeting 16S rRNA to identify the cecal microbial community. We designed a novel species-specific 16S rDNA probe to detect Lactobacillus rhamnosus (Lrham454). Subtractive technique using the LAC722 probe (Sakai et al. 2004 Journal of Bioscience and Bioengineering 98, 48) under different hybridization stringency (LAC722(L-H)) was applied to identify Lactococcus lactis. We also applied Lplan447 and LAC722(L) to detect Lactobacillus plantarum and a wide range of LAB (total LAB), respectively. We optimized the hybridization and washing conditions and then quantified L. rhamnosus, L. plantarum, and L. lactis cells in rat cecal contents. We monitored increases in individual bacterial populations and in total LAB caused by the administration of the corresponding LAB cells. Growth, food efficiency and internal disorders did not significantly differ among the rats administered with LABs. Rats administered with polydextrose (POL) developed diarrhea, which decreased the total numbers of cecal bacteria, whereas the simultaneous administration of POL and L. rhamnosus KY-3 eased this symptom, and recovered the numbers of total LAB and of L. rhamnosus.
UR - http://www.scopus.com/inward/record.url?scp=33646589001&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646589001&partnerID=8YFLogxK
U2 - 10.1007/s11274-005-9062-8
DO - 10.1007/s11274-005-9062-8
M3 - Article
VL - 22
SP - 493
EP - 499
JO - World Journal of Microbiology and Biotechnology
JF - World Journal of Microbiology and Biotechnology
SN - 0959-3993
IS - 5
ER -