Specific detection of Escherichia coli by using metallic mesh sensor in THz region

Ippei Kurita, Tetsuhito Suzuki, Naoshi Kondo, Takashi Kondo, Seiji Kamba, Yoshiko Miura, Yuichi Ogawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We demonstrated specific and rapid detection of Escherichia coli (E. coli) without culturing and labeling, using a metallic mesh sensor. Streptavidin and biotinylated antibody, specific for E. coli, were immobilized on the metallic mesh surface via amination and maleimidation. E. coli in PBS were captured, and attached to the surface of the metallic mesh, by immunoreaction with the antibody for E. coli. Transmission spectra of these samples were then measured around 10 THz, where the size of the generated, localized, electric field is the same as that of E. coli. After demonstrating a significant relationship between the frequency shift on the metallic mesh sensor and antibody for E. coli concentration, we then went on to specifically and quantitatively measure E. coli concentration. As E. coli concentration increased so did the spectral frequency shift, with detection limit being observed at 106 mL-1.

Original languageEnglish
Title of host publication2014 31th URSI General Assembly and Scientific Symposium, URSI GASS 2014
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781467352253
DOIs
Publication statusPublished - Oct 17 2014
Event31st General Assembly and Scientific Symposium of the International Union of Radio Science, URSI GASS 2014 - Beijing, China
Duration: Aug 16 2014Aug 23 2014

Publication series

Name2014 31th URSI General Assembly and Scientific Symposium, URSI GASS 2014

Other

Other31st General Assembly and Scientific Symposium of the International Union of Radio Science, URSI GASS 2014
CountryChina
CityBeijing
Period8/16/148/23/14

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering
  • Computer Networks and Communications

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