TY - JOUR
T1 - Specific p53 mutations predict poor prognosis in oral squamous cell carcinoma
AU - Yamazaki, Yutaka
AU - Chiba, Itsuo
AU - Hirai, Atsuko
AU - Sugiura, Chihiro
AU - Notani, Ken ichi
AU - Kashiwazaki, Haruhiko
AU - Tei, Kanchu
AU - Totsuka, Yasunori
AU - Fukuda, Hiroshi
PY - 2003/2
Y1 - 2003/2
N2 - In this study, we focused on p53 mutations in specific regions, including DNA-binding surface regions, to clarify the correlation between mutations within the specific regions of p53 and clinical outcomes of patients with oral cancers. We analyzed p53 mutations in 121 fresh primary oral squamous cell carcinomas (SCCs) by polymerase chain reaction-single-strand conformation polymorphism or a yeast functional assay. p53 mutations were detected in 51/121 (42%) cases. Mutation of p53 was not associated with any clinicopathological parameters; however, tumors containing specific p53 mutations, e.g. DNA-binding surface regions (L2, L3 and the LSH motif) and conserved regions (II-V), had significantly poorer prognoses than tumors with mutations outside of those regions. Moreover, locoregional failure, lymph node metastasis and the occurrence of subsequent distant metastasis were also significantly associated with mutations within DNA-binding surface regions. These data indicate that specific mutations of p53 could be important prognostic factors in oral SCCs.
AB - In this study, we focused on p53 mutations in specific regions, including DNA-binding surface regions, to clarify the correlation between mutations within the specific regions of p53 and clinical outcomes of patients with oral cancers. We analyzed p53 mutations in 121 fresh primary oral squamous cell carcinomas (SCCs) by polymerase chain reaction-single-strand conformation polymorphism or a yeast functional assay. p53 mutations were detected in 51/121 (42%) cases. Mutation of p53 was not associated with any clinicopathological parameters; however, tumors containing specific p53 mutations, e.g. DNA-binding surface regions (L2, L3 and the LSH motif) and conserved regions (II-V), had significantly poorer prognoses than tumors with mutations outside of those regions. Moreover, locoregional failure, lymph node metastasis and the occurrence of subsequent distant metastasis were also significantly associated with mutations within DNA-binding surface regions. These data indicate that specific mutations of p53 could be important prognostic factors in oral SCCs.
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U2 - 10.1016/S1368-8375(02)00064-7
DO - 10.1016/S1368-8375(02)00064-7
M3 - Article
C2 - 12509970
AN - SCOPUS:12244262241
VL - 39
SP - 163
EP - 169
JO - Oral Oncology
JF - Oral Oncology
SN - 1368-8375
IS - 2
ER -