Spectral assignments and anisotropy data of cellulose I: 13C-NMR chemical shift data of cellulose Iα determined by INADEQUATE and RAI techniques applied to uniformly 13C-labeled bacterial celluloses of different Gluconacetobacter xylinus strains

Stephanie Hesse-Ertelt, Raiker Witter, Anne S. Ulrich, Tetsuo Kondo, Thomas Heinze

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Solid-state 13C-NMR spectroscopy was used to characterize native cellulose pellicles from two strains of Gluconacetobacter xylinus (ATCC 53582, ATCC 23769), which had been statically cultivated in Hestrin-Schramm (HS) medium containing fully 13C-labeled β-D-glucose-U-13C 6 as the sole source of carbon. For both samples, the 13C-NMR chemical shifts were completely assigned for each 13C-labeled site of cellulose Iα with the aid of 2D refocused INADEQUATE NMR. To determine the principal chemical shift tensor components, a pulse sequence based on the recoupling of anisotropy information (RAI) was applied at 10 kHz MAS. The detailed 13C tensors of cellulose Iα from different bacterial celluloses are thus available now for the first time, and these results have been compared with previously published data of nonenriched material and with theoretical predictions.

Original languageEnglish
Pages (from-to)1030-1036
Number of pages7
JournalMagnetic Resonance in Chemistry
Volume46
Issue number11
DOIs
Publication statusPublished - Nov 1 2008

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)

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