Spectroscopical studies on the light-harvesting pigment-protein complex II from dark-aerobic and light-anaerobic grown cells of Rhodobacter sulfidophilus

Michio Doi, Yuzo Shioi, Nasser Gad'on, Jochen R. Golecki, Gerhart Drews

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The photosynthetic bacterium Rhodobacter sulfidophilus can grow and synthesize photosynthetic pigments under dark-aerobic as well as light-anaerobic growth conditions. Under both growth conditions intracytoplasmic membrane vesicles (diameter about 35 nm) are formed. The light-harvesting (LH) pigment-protein complex II, isolated from dark-aerobic and light-anaerobic grown cells, consists of two small polypeptides (< 5 kDa) and the pigments bacteriochlorophyll and carotenoids. The carotenoid composition of the LHII complex, as shown by absorption spectroscopy in the 450 to 550 nm region, changed when the cells were transferred from aerobic-dark to anaerobic-light conditions. The absorption maxima in the near infrared regions varied reversibly depending on such factors as salt concentration and detergent. These shifts of absorption bands either reflect a change of tertiary structure of the polypeptides, which then modify pigment-pigment and/or reflect a direct influence of the ions onto the conjugated system of the pigments.

Original languageEnglish
Pages (from-to)235-241
Number of pages7
JournalBBA - Bioenergetics
Volume1058
Issue number2
DOIs
Publication statusPublished - Jun 17 1991

Fingerprint

Rhodobacter
Light-Harvesting Protein Complexes
Pigments
Carotenoids
Light
Bacteriochlorophylls
Peptides
Proteins
Growth
Detergents
Spectrum Analysis
Salts
Ions
Bacteria
Membranes
Absorption spectroscopy
Absorption spectra
Infrared radiation
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Spectroscopical studies on the light-harvesting pigment-protein complex II from dark-aerobic and light-anaerobic grown cells of Rhodobacter sulfidophilus. / Doi, Michio; Shioi, Yuzo; Gad'on, Nasser; Golecki, Jochen R.; Drews, Gerhart.

In: BBA - Bioenergetics, Vol. 1058, No. 2, 17.06.1991, p. 235-241.

Research output: Contribution to journalArticle

Doi, Michio ; Shioi, Yuzo ; Gad'on, Nasser ; Golecki, Jochen R. ; Drews, Gerhart. / Spectroscopical studies on the light-harvesting pigment-protein complex II from dark-aerobic and light-anaerobic grown cells of Rhodobacter sulfidophilus. In: BBA - Bioenergetics. 1991 ; Vol. 1058, No. 2. pp. 235-241.
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