Spectroscopy-tomography of living single-cells

We propose the spectroscopy-tomography of single cells to improve the early detection and treatment of cancer. This technology can obtain the 3-dimensional distribution of components at a high spatial resolution. In this paper, we mention the analysis result of the cross-sectional images of the microsphere which diameter is 10 μm. The distribution of the internal submicron-defect in the microsphere can be analyzed. And to correct the center of rotation, we propose the image processing that uses the normalized correlation function as estimated value. The cross-sectional image of the microsphere is improved and the vague internal defect becomes to be distinguished by this proposed method. And we mention the proposed variable phase-contrast spectrometry as the 2- dimensional high spatial resolution spectrometry. This proposed method is a phase-shift interferometry between the 0th order diffracted light and the higher order diffracted light. We discuss the experimental results of the spectral characteristics using the proposed variable phase-contrast spectrometry. We measured the spectral characteristics at each pixel using the color filter of the liquid crystal and verified that the 2-dimensional spectral characteristics can be measured with good result.