Spherical parasporal inclusions of the Lepidoptera-specific and Coleoptera-specific Bacillus thuringiensis strains: A comparative electron microscopic study

Naoya Wasano, Chisa Yasunaga-Aoki, Ryoichi Sato, Michio Ohba, Takeshi Kawarabata, Hidenori Iwahana

Research output: Contribution to journalArticle

6 Citations (Scopus)


Four Lepidoptera-specific Bacillus thuringiensis strains that belong to the four H serogroups (serovars sumiyoshiensis, fukuokaensis, darmstadiensis, and japonensis) and a Coleoptera (Scarabaeidae)-specific strain belonging to serovar japonensis were examined for comparative ultrastructure of spherical parasporal inclusions. The prominent feature of the inclusions of the Lepidoptera-specific strains was the existence of thick, highly electron- dense envelopes surrounding a homogeneous protein matrix. The envelopes were 15.0-66.7 nm thick and consisted of 5-12 layers of membrane. This is also the case with inclusions of a Coleoptera-specific strain. The ultrastructure of inclusions from the five strains was in marked contrast to that of the bipyramidal parasporal inclusions produced by a Lepidoptera-specific serovar sotto strain.

Original languageEnglish
Pages (from-to)128-131
Number of pages4
JournalCurrent Microbiology
Issue number2
Publication statusPublished - Jan 18 2000


All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this