Spherical parasporal inclusions of the Lepidoptera-specific and Coleoptera-specific Bacillus thuringiensis strains: A comparative electron microscopic study

Naoya Wasano, Chisa Yasunaga-Aoki, Ryoichi Sato, Michio Ohba, Takeshi Kawarabata, Hidenori Iwahana

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Abstract

Four Lepidoptera-specific Bacillus thuringiensis strains that belong to the four H serogroups (serovars sumiyoshiensis, fukuokaensis, darmstadiensis, and japonensis) and a Coleoptera (Scarabaeidae)-specific strain belonging to serovar japonensis were examined for comparative ultrastructure of spherical parasporal inclusions. The prominent feature of the inclusions of the Lepidoptera-specific strains was the existence of thick, highly electron- dense envelopes surrounding a homogeneous protein matrix. The envelopes were 15.0-66.7 nm thick and consisted of 5-12 layers of membrane. This is also the case with inclusions of a Coleoptera-specific strain. The ultrastructure of inclusions from the five strains was in marked contrast to that of the bipyramidal parasporal inclusions produced by a Lepidoptera-specific serovar sotto strain.

Original languageEnglish
Pages (from-to)128-131
Number of pages4
JournalCurrent Microbiology
Volume40
Issue number2
DOIs
Publication statusPublished - Jan 18 2000

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

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