Sphingomyelin Stereoisomers Reveal That Homophilic Interactions Cause Nanodomain Formation

Yo Yano, Shinya Hanashima, Tomokazu Yasuda, Hiroshi Tsuchikawa, Nobuaki Matsumori, Masanao Kinoshita, Md Abdullah Al Sazzad, J. Peter Slotte, Michio Murata

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Sphingomyelin is an abundant lipid in some cellular membrane domains, such as lipid rafts. Hydrogen bonding and hydrophobic interactions of the lipid with surrounding components such as neighboring sphingomyelin and cholesterol (Cho) are widely considered to stabilize the raft-like liquid-ordered (Lo) domains in membrane bilayers. However, details of their interactions responsible for the formation of Lo domains remain largely unknown. In this study, the enantiomer of stearoyl sphingomyelin (ent-SSM) was prepared, and its physicochemical properties were compared with the natural SSM and the diastereomer of SSM to examine possible stereoselective lipid-lipid interactions. Interestingly, differential scanning calorimetry experiments demonstrated that palmitoyl sphingomyelin, with natural stereochemistry, exhibited higher miscibility with SSM bilayers than with ent-SSM bilayers, indicating that the homophilic sphingomyelin interactions occurred in a stereoselective manner. Solid-state 2H NMR revealed that Cho elicited its ordering effect very similarly on SSM and ent-SSM (and even on the diastereomer of SSM), suggesting that SSM-Cho interactions are not significantly affected by stereospecific hydrogen bonding. SSM and ent-SSM formed gel-like domains with very similar lateral packing in SSM/Cho/palmitoyloleoyl phosphatidylcholine membranes, as shown by fluorescence lifetime experiments. This observation can be explained by a homophilic hydrogen-bond network, which was largely responsible for the formation of gel-like nanodomains of SSMs (or ent-SSM). Our previous study revealed that Cho-poor gel-like domains contributed significantly to the formation of an Lo phase in sphingomyelin/Cho membranes. The results of the study presented here further show that SSM-SSM interactions occur near the headgroup region, whereas hydrophobic SSM-Cho interactions appeared important in the bilayer interior for Lo domain formation. The homophilic interactions of sphingomyelins could be mainly responsible for the formation of the domains of nanometer size, which may correspond to the small sphingomyelin/Cho-based rafts that temporally occur in biological membranes.

Original languageEnglish
Pages (from-to)1530-1540
Number of pages11
JournalBiophysical Journal
Volume115
Issue number8
DOIs
Publication statusPublished - Oct 16 2018

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Stereoisomerism
Sphingomyelins
Cholesterol
Lipids
Membranes
Gels
Hydrogen Bonding
Differential Scanning Calorimetry
Phosphatidylcholines
Hydrophobic and Hydrophilic Interactions
Hydrogen
Fluorescence

All Science Journal Classification (ASJC) codes

  • Biophysics

Cite this

Sphingomyelin Stereoisomers Reveal That Homophilic Interactions Cause Nanodomain Formation. / Yano, Yo; Hanashima, Shinya; Yasuda, Tomokazu; Tsuchikawa, Hiroshi; Matsumori, Nobuaki; Kinoshita, Masanao; Al Sazzad, Md Abdullah; Slotte, J. Peter; Murata, Michio.

In: Biophysical Journal, Vol. 115, No. 8, 16.10.2018, p. 1530-1540.

Research output: Contribution to journalArticle

Yano, Y, Hanashima, S, Yasuda, T, Tsuchikawa, H, Matsumori, N, Kinoshita, M, Al Sazzad, MA, Slotte, JP & Murata, M 2018, 'Sphingomyelin Stereoisomers Reveal That Homophilic Interactions Cause Nanodomain Formation', Biophysical Journal, vol. 115, no. 8, pp. 1530-1540. https://doi.org/10.1016/j.bpj.2018.08.042
Yano, Yo ; Hanashima, Shinya ; Yasuda, Tomokazu ; Tsuchikawa, Hiroshi ; Matsumori, Nobuaki ; Kinoshita, Masanao ; Al Sazzad, Md Abdullah ; Slotte, J. Peter ; Murata, Michio. / Sphingomyelin Stereoisomers Reveal That Homophilic Interactions Cause Nanodomain Formation. In: Biophysical Journal. 2018 ; Vol. 115, No. 8. pp. 1530-1540.
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AU - Matsumori, Nobuaki

AU - Kinoshita, Masanao

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