Sphingosine-1-phosphate-enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2 cells

Yoko Hashimoto, Mari Kobayashi, Etsuko Matsuzaki, Katsumasa Higashi, Fumi Takahashi-Yanaga, Aiko Takano, Masato Hirata, Fusanori Nishimura

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In this study, we investigated the involvement of Wnt signaling in sphingosine-1-phosphate (S1P)-enhanced osteogenic differentiation of C3H10T1/2 pluripotent stem cells. We found that S1P enhanced the expression of Wnt5a and low-density lipoprotein receptor-related protein 5 or 6 (LRP5/6) during osteogenic differentiation. Wnt5a-neutralizing antibody inhibited S1P-enhanced expression of LRP5/6 and alkaline phosphatase, which are essential for osteogenic differentiation. Conversely, S1P did not affect endogenous canonical Wnt signaling. Taken together, S1P-enhanced Wnt5a promotes LRP5/6 expression, resulting in the trigger of osteogenic differentiation of C3H10T1/2 cells. These findings suggest a potential beneficial role for S1P in bone regeneration.

Original languageEnglish
Pages (from-to)1129-1136
Number of pages8
JournalCell Biology International
Volume40
Issue number10
DOIs
Publication statusPublished - Oct 1 2016

All Science Journal Classification (ASJC) codes

  • Cell Biology

Fingerprint Dive into the research topics of 'Sphingosine-1-phosphate-enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2 cells'. Together they form a unique fingerprint.

  • Cite this

    Hashimoto, Y., Kobayashi, M., Matsuzaki, E., Higashi, K., Takahashi-Yanaga, F., Takano, A., Hirata, M., & Nishimura, F. (2016). Sphingosine-1-phosphate-enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2 cells. Cell Biology International, 40(10), 1129-1136. https://doi.org/10.1002/cbin.10652