Sphingosine-1-phosphate-enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2 cells

Yoko Hashimoto, Mari Kobayashi, Etsuko Matsuzaki, Katsumasa Higashi, Fumi Takahashi-Yanaga, Aiko Takano, Masato Hirata, Fusanori Nishimura

Research output: Contribution to journalArticle

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Abstract

In this study, we investigated the involvement of Wnt signaling in sphingosine-1-phosphate (S1P)-enhanced osteogenic differentiation of C3H10T1/2 pluripotent stem cells. We found that S1P enhanced the expression of Wnt5a and low-density lipoprotein receptor-related protein 5 or 6 (LRP5/6) during osteogenic differentiation. Wnt5a-neutralizing antibody inhibited S1P-enhanced expression of LRP5/6 and alkaline phosphatase, which are essential for osteogenic differentiation. Conversely, S1P did not affect endogenous canonical Wnt signaling. Taken together, S1P-enhanced Wnt5a promotes LRP5/6 expression, resulting in the trigger of osteogenic differentiation of C3H10T1/2 cells. These findings suggest a potential beneficial role for S1P in bone regeneration.

Original languageEnglish
Pages (from-to)1129-1136
Number of pages8
JournalCell Biology International
Volume40
Issue number10
DOIs
Publication statusPublished - Oct 1 2016

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Low Density Lipoprotein Receptor-Related Protein-6
Low Density Lipoprotein Receptor-Related Protein-5
Pluripotent Stem Cells
Bone Regeneration
Neutralizing Antibodies
Alkaline Phosphatase
sphingosine 1-phosphate

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Hashimoto, Y., Kobayashi, M., Matsuzaki, E., Higashi, K., Takahashi-Yanaga, F., Takano, A., ... Nishimura, F. (2016). Sphingosine-1-phosphate-enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2 cells. Cell Biology International, 40(10), 1129-1136. https://doi.org/10.1002/cbin.10652

Sphingosine-1-phosphate-enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2 cells. / Hashimoto, Yoko; Kobayashi, Mari; Matsuzaki, Etsuko; Higashi, Katsumasa; Takahashi-Yanaga, Fumi; Takano, Aiko; Hirata, Masato; Nishimura, Fusanori.

In: Cell Biology International, Vol. 40, No. 10, 01.10.2016, p. 1129-1136.

Research output: Contribution to journalArticle

Hashimoto, Y, Kobayashi, M, Matsuzaki, E, Higashi, K, Takahashi-Yanaga, F, Takano, A, Hirata, M & Nishimura, F 2016, 'Sphingosine-1-phosphate-enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2 cells', Cell Biology International, vol. 40, no. 10, pp. 1129-1136. https://doi.org/10.1002/cbin.10652
Hashimoto Y, Kobayashi M, Matsuzaki E, Higashi K, Takahashi-Yanaga F, Takano A et al. Sphingosine-1-phosphate-enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2 cells. Cell Biology International. 2016 Oct 1;40(10):1129-1136. https://doi.org/10.1002/cbin.10652
Hashimoto, Yoko ; Kobayashi, Mari ; Matsuzaki, Etsuko ; Higashi, Katsumasa ; Takahashi-Yanaga, Fumi ; Takano, Aiko ; Hirata, Masato ; Nishimura, Fusanori. / Sphingosine-1-phosphate-enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2 cells. In: Cell Biology International. 2016 ; Vol. 40, No. 10. pp. 1129-1136.
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