Sphingosine 1-phosphate receptors mediate stimulatory and inhibitory signalings for expression of adhesion molecules in endothelial cells

Takao Kimura, Hideaki Tomura, Chihiro Mogi, Atsushi Kuwabara, Mitsuteru Ishiwara, Kunihiko Shibasawa, Koichi Sato, Susumu Ohwada, Doon Soon Im, Hitoshi Kurose, Tamotsu Ishizuka, Masami Murakami, Fumikazu Okajima

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Sphingosine 1-phosphate (S1P) stimulates expression of vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 in human umbilical vein endothelial cells. S1P-induced actions were associated with nuclear factor kappa-B activation and inhibited by pertussis toxin as well as by antisense oligonucleotides specific to S1P receptors, especially, S1P3. S1P also stimulated endothelial nitric oxide synthase (eNOS) and its activation was markedly inhibited by the antisense oligonucleotide for the S1P1 receptor rather than that for the S1P3 receptor. The dose-response curve of S1P to stimulate adhesion molecule expression was shifted to the left in the presence of the phosphatidylinositol 3-kinase inhibitor wortmannin and the NOS inhibitor Nω-nitro-l-arginine methyl ester. NO donor S-nitroso-N-acetylpenicillamine inhibited S1P-induced adhesion molecule expression. Moreover, tumor necrosis factor-α-induced adhesion molecule expression was markedly inhibited by S1P in a manner sensitive to inhibitors for PI3-K and NOS. These results suggest that S1P receptors are coupled to both stimulatory and inhibitory pathways for adhesion molecule expression. The stimulatory pathway involves nuclear factor kappa-B and inhibitory one does phosphatidylinositol 3-kinase and NOS.

Original languageEnglish
Pages (from-to)841-850
Number of pages10
JournalCellular Signalling
Volume18
Issue number6
DOIs
Publication statusPublished - Jun 1 2006

Fingerprint

Lysosphingolipid Receptors
Endothelial Cells
Phosphatidylinositol 3-Kinase
NF-kappa B
Antisense Oligonucleotides
S-Nitroso-N-Acetylpenicillamine
Vascular Cell Adhesion Molecule-1
Nitric Oxide Synthase Type III
Pertussis Toxin
Human Umbilical Vein Endothelial Cells
Intercellular Adhesion Molecule-1
sphingosine 1-phosphate
Tumor Necrosis Factor-alpha

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Sphingosine 1-phosphate receptors mediate stimulatory and inhibitory signalings for expression of adhesion molecules in endothelial cells. / Kimura, Takao; Tomura, Hideaki; Mogi, Chihiro; Kuwabara, Atsushi; Ishiwara, Mitsuteru; Shibasawa, Kunihiko; Sato, Koichi; Ohwada, Susumu; Im, Doon Soon; Kurose, Hitoshi; Ishizuka, Tamotsu; Murakami, Masami; Okajima, Fumikazu.

In: Cellular Signalling, Vol. 18, No. 6, 01.06.2006, p. 841-850.

Research output: Contribution to journalArticle

Kimura, T, Tomura, H, Mogi, C, Kuwabara, A, Ishiwara, M, Shibasawa, K, Sato, K, Ohwada, S, Im, DS, Kurose, H, Ishizuka, T, Murakami, M & Okajima, F 2006, 'Sphingosine 1-phosphate receptors mediate stimulatory and inhibitory signalings for expression of adhesion molecules in endothelial cells', Cellular Signalling, vol. 18, no. 6, pp. 841-850. https://doi.org/10.1016/j.cellsig.2005.07.011
Kimura, Takao ; Tomura, Hideaki ; Mogi, Chihiro ; Kuwabara, Atsushi ; Ishiwara, Mitsuteru ; Shibasawa, Kunihiko ; Sato, Koichi ; Ohwada, Susumu ; Im, Doon Soon ; Kurose, Hitoshi ; Ishizuka, Tamotsu ; Murakami, Masami ; Okajima, Fumikazu. / Sphingosine 1-phosphate receptors mediate stimulatory and inhibitory signalings for expression of adhesion molecules in endothelial cells. In: Cellular Signalling. 2006 ; Vol. 18, No. 6. pp. 841-850.
@article{72cb2265212f4950a9d2b575c5a8acb5,
title = "Sphingosine 1-phosphate receptors mediate stimulatory and inhibitory signalings for expression of adhesion molecules in endothelial cells",
abstract = "Sphingosine 1-phosphate (S1P) stimulates expression of vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 in human umbilical vein endothelial cells. S1P-induced actions were associated with nuclear factor kappa-B activation and inhibited by pertussis toxin as well as by antisense oligonucleotides specific to S1P receptors, especially, S1P3. S1P also stimulated endothelial nitric oxide synthase (eNOS) and its activation was markedly inhibited by the antisense oligonucleotide for the S1P1 receptor rather than that for the S1P3 receptor. The dose-response curve of S1P to stimulate adhesion molecule expression was shifted to the left in the presence of the phosphatidylinositol 3-kinase inhibitor wortmannin and the NOS inhibitor Nω-nitro-l-arginine methyl ester. NO donor S-nitroso-N-acetylpenicillamine inhibited S1P-induced adhesion molecule expression. Moreover, tumor necrosis factor-α-induced adhesion molecule expression was markedly inhibited by S1P in a manner sensitive to inhibitors for PI3-K and NOS. These results suggest that S1P receptors are coupled to both stimulatory and inhibitory pathways for adhesion molecule expression. The stimulatory pathway involves nuclear factor kappa-B and inhibitory one does phosphatidylinositol 3-kinase and NOS.",
author = "Takao Kimura and Hideaki Tomura and Chihiro Mogi and Atsushi Kuwabara and Mitsuteru Ishiwara and Kunihiko Shibasawa and Koichi Sato and Susumu Ohwada and Im, {Doon Soon} and Hitoshi Kurose and Tamotsu Ishizuka and Masami Murakami and Fumikazu Okajima",
year = "2006",
month = "6",
day = "1",
doi = "10.1016/j.cellsig.2005.07.011",
language = "English",
volume = "18",
pages = "841--850",
journal = "Cellular Signalling",
issn = "0898-6568",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Sphingosine 1-phosphate receptors mediate stimulatory and inhibitory signalings for expression of adhesion molecules in endothelial cells

AU - Kimura, Takao

AU - Tomura, Hideaki

AU - Mogi, Chihiro

AU - Kuwabara, Atsushi

AU - Ishiwara, Mitsuteru

AU - Shibasawa, Kunihiko

AU - Sato, Koichi

AU - Ohwada, Susumu

AU - Im, Doon Soon

AU - Kurose, Hitoshi

AU - Ishizuka, Tamotsu

AU - Murakami, Masami

AU - Okajima, Fumikazu

PY - 2006/6/1

Y1 - 2006/6/1

N2 - Sphingosine 1-phosphate (S1P) stimulates expression of vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 in human umbilical vein endothelial cells. S1P-induced actions were associated with nuclear factor kappa-B activation and inhibited by pertussis toxin as well as by antisense oligonucleotides specific to S1P receptors, especially, S1P3. S1P also stimulated endothelial nitric oxide synthase (eNOS) and its activation was markedly inhibited by the antisense oligonucleotide for the S1P1 receptor rather than that for the S1P3 receptor. The dose-response curve of S1P to stimulate adhesion molecule expression was shifted to the left in the presence of the phosphatidylinositol 3-kinase inhibitor wortmannin and the NOS inhibitor Nω-nitro-l-arginine methyl ester. NO donor S-nitroso-N-acetylpenicillamine inhibited S1P-induced adhesion molecule expression. Moreover, tumor necrosis factor-α-induced adhesion molecule expression was markedly inhibited by S1P in a manner sensitive to inhibitors for PI3-K and NOS. These results suggest that S1P receptors are coupled to both stimulatory and inhibitory pathways for adhesion molecule expression. The stimulatory pathway involves nuclear factor kappa-B and inhibitory one does phosphatidylinositol 3-kinase and NOS.

AB - Sphingosine 1-phosphate (S1P) stimulates expression of vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 in human umbilical vein endothelial cells. S1P-induced actions were associated with nuclear factor kappa-B activation and inhibited by pertussis toxin as well as by antisense oligonucleotides specific to S1P receptors, especially, S1P3. S1P also stimulated endothelial nitric oxide synthase (eNOS) and its activation was markedly inhibited by the antisense oligonucleotide for the S1P1 receptor rather than that for the S1P3 receptor. The dose-response curve of S1P to stimulate adhesion molecule expression was shifted to the left in the presence of the phosphatidylinositol 3-kinase inhibitor wortmannin and the NOS inhibitor Nω-nitro-l-arginine methyl ester. NO donor S-nitroso-N-acetylpenicillamine inhibited S1P-induced adhesion molecule expression. Moreover, tumor necrosis factor-α-induced adhesion molecule expression was markedly inhibited by S1P in a manner sensitive to inhibitors for PI3-K and NOS. These results suggest that S1P receptors are coupled to both stimulatory and inhibitory pathways for adhesion molecule expression. The stimulatory pathway involves nuclear factor kappa-B and inhibitory one does phosphatidylinositol 3-kinase and NOS.

UR - http://www.scopus.com/inward/record.url?scp=32144457352&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=32144457352&partnerID=8YFLogxK

U2 - 10.1016/j.cellsig.2005.07.011

DO - 10.1016/j.cellsig.2005.07.011

M3 - Article

C2 - 16111867

AN - SCOPUS:32144457352

VL - 18

SP - 841

EP - 850

JO - Cellular Signalling

JF - Cellular Signalling

SN - 0898-6568

IS - 6

ER -