Sphingosine kinases and their metabolites modulate endolysosomal trafficking in photoreceptors

Ikuko Yonamine, Takeshi Bamba, Niraj K. Nirala, Nahid Jesmin, Teresa Kosakowska-Cholody, Kunio Nagashima, Eiichiro Fukusaki, Jairaj K. Acharya, Usha Acharya

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Internalized membrane proteins are either transported to late endosomes and lysosomes for degradation or recycled to the plasma membrane. Although proteins involved in trafficking and sorting have been well studied, far less is known about the lipid molecules that regulate the intracellular trafficking of membrane proteins. We studied the function of sphingosine kinases and their metabolites in endosomal trafficking using Drosophila melanogaster photoreceptors as a model system. Gain- and loss-of-function analyses show that sphingosine kinases affect trafficking of the G protein-coupled receptor Rhodopsin and the light-sensitive transient receptor potential (TRP) channel by modulating the levels of dihydrosphingosine 1 phosphate (DHS1P) and sphingosine 1 phosphate (S1P). An increase in DHS1P levels relative to S1P leads to the enhanced lysosomal degradation of Rhodopsin and TRP and retinal degeneration in wild-type photoreceptors. Our results suggest that sphingosine kinases and their metabolites modulate photoreceptor homeostasis by influencing endolysosomal trafficking of Rhodopsin and TRP.

Original languageEnglish
Pages (from-to)557-567
Number of pages11
JournalJournal of Cell Biology
Volume192
Issue number4
DOIs
Publication statusPublished - Feb 21 2011

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Rhodopsin
Membrane Proteins
Transient Receptor Potential Channels
Retinal Degeneration
Intracellular Membranes
Endosomes
G-Protein-Coupled Receptors
Lysosomes
Drosophila melanogaster
Homeostasis
Cell Membrane
Lipids
Light
sphingosine kinase
Proteins
sphingosine 1-phosphate
dihydrosphingosine 1-phosphate

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Yonamine, I., Bamba, T., Nirala, N. K., Jesmin, N., Kosakowska-Cholody, T., Nagashima, K., ... Acharya, U. (2011). Sphingosine kinases and their metabolites modulate endolysosomal trafficking in photoreceptors. Journal of Cell Biology, 192(4), 557-567. https://doi.org/10.1083/jcb.201004098

Sphingosine kinases and their metabolites modulate endolysosomal trafficking in photoreceptors. / Yonamine, Ikuko; Bamba, Takeshi; Nirala, Niraj K.; Jesmin, Nahid; Kosakowska-Cholody, Teresa; Nagashima, Kunio; Fukusaki, Eiichiro; Acharya, Jairaj K.; Acharya, Usha.

In: Journal of Cell Biology, Vol. 192, No. 4, 21.02.2011, p. 557-567.

Research output: Contribution to journalArticle

Yonamine, I, Bamba, T, Nirala, NK, Jesmin, N, Kosakowska-Cholody, T, Nagashima, K, Fukusaki, E, Acharya, JK & Acharya, U 2011, 'Sphingosine kinases and their metabolites modulate endolysosomal trafficking in photoreceptors', Journal of Cell Biology, vol. 192, no. 4, pp. 557-567. https://doi.org/10.1083/jcb.201004098
Yonamine, Ikuko ; Bamba, Takeshi ; Nirala, Niraj K. ; Jesmin, Nahid ; Kosakowska-Cholody, Teresa ; Nagashima, Kunio ; Fukusaki, Eiichiro ; Acharya, Jairaj K. ; Acharya, Usha. / Sphingosine kinases and their metabolites modulate endolysosomal trafficking in photoreceptors. In: Journal of Cell Biology. 2011 ; Vol. 192, No. 4. pp. 557-567.
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