Spinning of a gigantic bundle of hollow fibrils by a spirally moving higher plant protoplast

Tomoko Seyama, Satoshi Kimura, Hamako Sasamoto, Hisashi Abe, Tetsuo Kondo

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A unique fiber spinning was found in protoplasts from white birch (Betula platyphylla) leaves under an acidic medium containing high concentration of Ca2+. After expanding from 10 to 100 μm in diameter under the culture condition, the protoplast started secreting a gigantic fiber while moving in a spiral way. Real time video analyses elucidated that the orientation, rate and pattern of the motion were directed due to the inverse force of the fiber spinning. Moreover, observation using several microscopic methods accompanied with histochemical staining and nuclear magnetic resonance (NMR) analysis indicated that the fiber was composed of 400-500 nm wide (1→3)-β-glucan hollow sub-fibrils. This entire phenomenon may be a response against the stress imposed. The observation presented provides an understanding of the unique relationship between fiber spinning and the bottom-up fiber fabrication from nano to micro scales.

Original languageEnglish
Pages (from-to)1187-1197
Number of pages11
JournalPlanta
Volume227
Issue number6
DOIs
Publication statusPublished - May 1 2008

Fingerprint

Betula
Protoplasts
spinning
protoplasts
Observation
Glucans
Magnetic Resonance Spectroscopy
glucans
Staining and Labeling
nuclear magnetic resonance spectroscopy
stress response
calcium
leaves
methodology

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science

Cite this

Spinning of a gigantic bundle of hollow fibrils by a spirally moving higher plant protoplast. / Seyama, Tomoko; Kimura, Satoshi; Sasamoto, Hamako; Abe, Hisashi; Kondo, Tetsuo.

In: Planta, Vol. 227, No. 6, 01.05.2008, p. 1187-1197.

Research output: Contribution to journalArticle

Seyama, Tomoko ; Kimura, Satoshi ; Sasamoto, Hamako ; Abe, Hisashi ; Kondo, Tetsuo. / Spinning of a gigantic bundle of hollow fibrils by a spirally moving higher plant protoplast. In: Planta. 2008 ; Vol. 227, No. 6. pp. 1187-1197.
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