Splicing in Caenorhabditis elegans does not require an AG at the 3' splice acceptor site

R. V. Aroian, A. D. Levy, M. Koga, Y. Ohshima, J. M. Kramer, P. W. Sternberg

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The dinucleotide AG, found at the 3' end of virtually all eukaryotic pre- mRNA introns, is thought to be essential for splicing. Reduction-of-function mutations in two Caenorhabditis elegans genes, the receptor tyrosine kinase gene let-23 and the collagen gene dpy-10, both alter the AG at the end of a short (ca. 50-nucleotide) intron to AA. The in vivo effects of these mutations were studied by sequencing polymerase chain reaction-amplified reverse-transcribed RNA isolated from the two mutants. As expected, we find transcripts that splice to a cryptic AG, skip an exon, and retain an unspliced intron. However, we also find significant levels of splicing at the mutated 3' splice site (AA) and at nearby non-AG dinucleotides. Our results indicate that for short C. elegans introns an AG is not required for splicing at either the correct 3' splice site or incorrect sites. Analysis of a splice site mutant involving a longer, 316-nucleotide C. elegans intron indicates that an AG is also not required there for splicing. We hypothesize that elements besides the invariant AG, e.g., an A-U-rich region, a UUUC motif, and/or a potential branch point sequence, are directing the selection of the 3' splice site and that in wild-type genes these elements cooperate so that proper splicing occurs.

Original languageEnglish
Pages (from-to)626-637
Number of pages12
JournalMolecular and cellular biology
Volume13
Issue number1
DOIs
Publication statusPublished - Jan 1 1993
Externally publishedYes

Fingerprint

RNA Splice Sites
Caenorhabditis elegans
Introns
Genes
Nucleotides
Mutation
RNA Precursors
Receptor Protein-Tyrosine Kinases
Exons
Collagen
RNA
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Splicing in Caenorhabditis elegans does not require an AG at the 3' splice acceptor site. / Aroian, R. V.; Levy, A. D.; Koga, M.; Ohshima, Y.; Kramer, J. M.; Sternberg, P. W.

In: Molecular and cellular biology, Vol. 13, No. 1, 01.01.1993, p. 626-637.

Research output: Contribution to journalArticle

Aroian, R. V. ; Levy, A. D. ; Koga, M. ; Ohshima, Y. ; Kramer, J. M. ; Sternberg, P. W. / Splicing in Caenorhabditis elegans does not require an AG at the 3' splice acceptor site. In: Molecular and cellular biology. 1993 ; Vol. 13, No. 1. pp. 626-637.
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