Spontaneous seizures in a rat models of multiple prenatal lesioning

Takashi Kamada, Kei Ichiro Takase, Hiroshi Shigeto

Research output: Contribution to journalArticle

Abstract

Focal cortical dysplasia (FCD) is an important cause of intractable epilepsy. Previous rat studies have utilized freeze lesioning of neonatal animals to model FCD; however, such models are unable to demonstrate spontaneous seizures without seizure-provoking events. Therefore, we created an animal model with multiple FCD, produced during embryonic development, and observed whether spontaneous seizures occurred. Furthermore, we examined the relationship between FCD and epileptogenesis using immunohistochemistry. At 18 days postconception, a frozen metal probe was placed bilaterally on the scalps of Sprague-Dawley rat embryos through the uterus wall to produce multiple FCD. Eleven of 16 rats showed spontaneous seizures arising in the hippocampus from postnatal day47. Movement cessation followed by sniffing and mastication, culminating in wet-dog shaking, was seen during the hippocampal EEG discharges. Alterations in the levels of glutamatergic and GABA-ergic receptors were investigated during growth. We created an animal model showing spontaneous seizures without a provoking event except for the existence of cortical dysplasia, and without a genetic or general systematic cause like MAM injection or irradiation. The seizures resembled human temporal lobe epilepsy both clinically and on EEG. This model should enable better clarification of the mechanisms underlying the development of human epilepsy.

Original languageEnglish
Pages (from-to)1132-1135
Number of pages4
JournalClinical Neurology
Volume54
Issue number12
DOIs
Publication statusPublished - Jan 1 2014

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Malformations of Cortical Development
Seizures
Animal Models
Electroencephalography
Newborn Animals
GABA Receptors
Temporal Lobe Epilepsy
Mastication
Human Development
Scalp
Uterus
Embryonic Development
Sprague Dawley Rats
Epilepsy
Hippocampus
Embryonic Structures
Metals
Immunohistochemistry
Dogs
Injections

All Science Journal Classification (ASJC) codes

  • Clinical Neurology

Cite this

Spontaneous seizures in a rat models of multiple prenatal lesioning. / Kamada, Takashi; Takase, Kei Ichiro; Shigeto, Hiroshi.

In: Clinical Neurology, Vol. 54, No. 12, 01.01.2014, p. 1132-1135.

Research output: Contribution to journalArticle

Kamada, Takashi ; Takase, Kei Ichiro ; Shigeto, Hiroshi. / Spontaneous seizures in a rat models of multiple prenatal lesioning. In: Clinical Neurology. 2014 ; Vol. 54, No. 12. pp. 1132-1135.
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