Spontaneous transmitter release recruits postsynaptic mechanisms of long-term and intermediate-term facilitation in Aplysia

Iksung Jin, Hiroshi Udo, Joseph B. Rayman, Sathya Puthanveettil, Eric R. Kandel, Robert D. Hawkins

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Whereas short-term (minutes) facilitation at Aplysia sensory-motor neuron synapses is presynaptic, long-term (days) facilitation involves synaptic growth, which requires both presynaptic and postsynaptic mechanisms. How are the postsynaptic mechanisms recruited, and when does that process begin? We have been investigating the possible role of spontaneous transmitter release from the presynaptic neuron. In the previous paper, we found that spontaneous release is critical for the induction of long-term facilitation, and this process begins during an intermediate-term stage of facilitation that is the first stage to involve postsynaptic as well as presynaptic mechanisms. We now report that increased spontaneous release during the short-term stage acts as an orthograde signal to recruit postsynaptic mechanisms of intermediate-term facilitation including increased IP3, Ca2+, and membrane insertion and recruitment of clusters of AMPA-like receptors, which may be first steps in synaptic growth during long-term facilitation. These results suggest that the different stages of facilitation involve a cascade of pre- and postsynaptic mechanisms, which is initiated by spontaneous release and may culminate in synaptic growth.

Original languageEnglish
Pages (from-to)9137-9142
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number23
DOIs
Publication statusPublished - Jun 5 2012

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Aplysia
Growth
AMPA Receptors
Motor Neurons
Sensory Receptor Cells
Synapses
Neurons
Membranes

All Science Journal Classification (ASJC) codes

  • General

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Spontaneous transmitter release recruits postsynaptic mechanisms of long-term and intermediate-term facilitation in Aplysia. / Jin, Iksung; Udo, Hiroshi; Rayman, Joseph B.; Puthanveettil, Sathya; Kandel, Eric R.; Hawkins, Robert D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 23, 05.06.2012, p. 9137-9142.

Research output: Contribution to journalArticle

Jin, Iksung ; Udo, Hiroshi ; Rayman, Joseph B. ; Puthanveettil, Sathya ; Kandel, Eric R. ; Hawkins, Robert D. / Spontaneous transmitter release recruits postsynaptic mechanisms of long-term and intermediate-term facilitation in Aplysia. In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 23. pp. 9137-9142.
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